[1108] Follow-Up Outcomes in a Large Patient Cohort with HPV-Negative ASC-H Computer-Imaged Liquid-Based Cytology (LBC) Results

David A Cohen, R Marshall Austin, Christopher Gilbert, Richard Freij, Chengquan Zhao. Magee-Womens Hospital of University of Pittsburgh Medical Center (UPMC), Pittsburgh, PA

Background: Adjunctive HPV testing with co-testing-based risk stratification has become increasingly common. Current guidelines recommend a colposcopy referral for women with ASC-H cytology regardless of HPV status. To the best of our knowledge, no large follow-up data has been published in women with ASC-H cytology and negative HPV test results.
Design: Patients with ASC-H ThinPrep LBC and negative HC2 HPV test results were retrieved from our database between June 2005 and December 2010. Cytology specimens were screened with computer-assistance by the ThinPrep Imaging System (TIS). Cytologic, histopathologic, and repeat HPV test follow-up results were analyzed.
Results: 1049 negative hrHPV and ASC-H LBC cases were identified. Patients without repeat cytologic testing or histopathologic follow-up results in our database were excluded from the study leaving 912 patients with an average age of 37.4 years (17-89). The average follow-up period was 29 months (1-73). Histopathologic and cytologic follow-up results are shown in Table 1. Eighteen (2.0%) patients subsequently developed CIN2/3 or cytologic HSIL. 208 (22.8%) patients developed histopathologic CIN1 or cytologic LSIL.
The average time to diagnosis for CIN2/3 cases was 10 months (range 1-40). 384 (42%) patients had a Pap test as initial follow-up with an average interval of 10 months (1-57). 470 (51.5%) patients had repeat hrHPV testing of which 45 (9.6%) had hrHPV-positive results (Table 2). An initial repeat hrHPV test was positive in 35 of 470 (7.4%) of patients. The average follow-up interval to an initial repeat hrHPV test was 16 months (1-64) and 22 months (1-62) to an initial repeat positive hrHPV test. The average number of repeat hrHPV tests was 1.5 (1-6 range).

Summary of Histologic and/or cytologic Follow-up Results

Follow-up Method(s)	Total Cases	CIN2/3/HSIL#	CIN1/LSIL
Histology	570	17(3.0)	194(34)
Cytology only	342	1(0.3)	14(4.1)
Total	912	18(2.0)	208(22.8)

Repeat hrHPV testing results

HPV testing	Cases (%)
Positive only	26 (5.5)
Negative only	425 (90.4)
Both positive and negative	19 (4.1)
Total	470 (100)

Conclusions: Our data, the largest study size to date, shows a very low incidence of CIN2/3 among women in this subgroup. Females with ASC-H Pap tests and negative hrHPV testing may be more efficiently managed by regular Pap and hrHPV testing rather than universal colposcopy. Additional long term follow-up studies are needed on patients with hrHPV testing and divergent cytology results utilizing new methods of cytology screening.
Category: Gynecologic & Obstetrics

Wednesday, March 21, 2012 1:00 PM

Poster Session VI # 190, Wednesday Afternoon