microRNA Expression Profiling of Low-Grade Endometrial Stromal Sarcomas and Undifferentiated Endometrial Sarcomas
Sarah Chiang, Maria A Castilla, Jose Palacios, Esther Oliva. Massachusetts General Hospital, Boston, MA; Hospital Universitario Virgen del Rocio-Instituto de Investigaciones Biomedicas, Sevilla, Spain
Background: microRNAs (miRNAs) are small, non-coding transcripts that regulate gene expression and may contribute to tumor development, progression, and metastasis. The aim of this study is to analyze miRNA signatures in endometrial stromal sarcomas (ESSs) with and without known gene rearrangements and undifferentiated endometrial sarcomas (UESs).
Design: Real-time PCR was used to acquire genome-wide miRNA expression profiles of 9 and 10 ESSs with and without rearrangements of JAZF1, SUZ12, EPC1, or PHF1 genes, respectively, and 4 UESs. miRNA signatures were compared between all ESSs and UESs and between ESSs with and without gene rearrangement.
Results: Similar miRNA expression profiles were observed in ESSs with and without gene rearrangement. However, 3 miRNAs (miR-203, -31, and -383) were upregulated and 6 (miR-224, -331-5p, -411, -486, -671-3p, and -197) were downregulated in tumors harboring known gene rearrangements. Fifteen miRNAs (miR-139-5p, -455-5p, -22, -10b, -183, -34a, -337-5p, -452, -187, -224, -187, -224, -886-5p, -379, -126, -193a-5p, and -744) were downregulated and 3 (miR-489, -518b, and -10a) were upregulated in UESs compared to ESSs. Analysis of miRNA-target gene pathways showed that several miRNAs altered in UESs are involved in WNT, VEGFR, and EGFR signaling pathways.
Conclusions: ESSs with and without gene rearrangement have similar miRNA expression profiles. WNT, VEGFR, and EGFR signaling pathways may be implicated in UES tumorigenesis, suggesting that UESs and low-grade ESSs may be related as both share WNT and EGFR signaling pathways.
Category: Gynecologic & Obstetrics
Tuesday, March 20, 2012 9:30 AM
Poster Session III # 185, Tuesday Morning