Precise Precursor Frequency Assessment by Digital Quantification of Oviductal Epithelium
Jonathan G Bijron, Gang Ning, Anna R Laury, Charles M Quick, Nicolas M Monte, Frank D McKeon, Wa Xian, Emily King, Christopher P Crum. Brigham and Women's Hospital, Boston, MA; Harvard Medical School, Boston, MA; UCLA Medical Center, Los Angeles, CA; University of Arkansas Medical Center, Little Rock, AR; Genome Institute, A*STAR, Singapore
Background: Presumed clonal expansions of secretory-type cells in fallopian tube epithelium include so-called secretory cell outgrowths (SCOUTs) that may be p53 positive (p53 signatures) or contain wild type p53. These entities have been associated with loss of PAX2 expression and, using tissue sections as a denominator, estimated to be more common in older women and in women with pelvic serous cancer. However, precise assessment requires determining frequency as a function of the surface area in the oviduct, which varies considerably from section to section. We have created a high throughput, low cost method for quantifying total epithelial length in microns using virtual slide images.
Design: Fallopian tube sections were selected from 35 patients with serous ovarian cancer and 35 controls. Each cross section was digitalised using an iScan brightfield scanner (BioImagene) and a scale bar was added. The images were uploaded in Adobe® Photoshop® CS3 Extended. Subsequently, pixel length was translated into microns and epithelial length measured using primarily the built in Magic Wand tool (Figure 1). PAX2-null SCOUTs were counted (arrows) and expressed as a function of overall surface area.
Results: The mean SCOUT incidence per micron was 3.88E-06 in cancer associated tubes and 1.28E-06 in control tubes (p=0.007). SCOUT incidence was significantly correlated with age, when divided in 4 age groups (36-45, 46-55, 56-65 and 66-7) the incidence increased with age in both cancer and control tubes (p=0.01).
Conclusions: The described method has verified higher SCOUT frequency in cancer-associated fallopian tubes and increases with advancing age. The described method of assessment can be used for measuring total epithelial length in a variety of tissues and is suited for both H&E and immunohistochemical staining with sufficiently high contrast. The total epithelial length can be used to more accurately assess and compare surface areas at risk and assign greater meaning to the numbers of lesions detected.
Category: Gynecologic & Obstetrics
Tuesday, March 20, 2012 9:30 AM
Poster Session III # 188, Tuesday Morning