GATA3, p63 and S100P: An IHC Comparison Analysis in Bladder Cancer
David Tacha, Ryan Bremer, Charlie Yu, Liang Chen. Biocare Medical, Concord, CA; Indiana University School of Medicine, Indianapolis, IN
Background: More than 90% of bladder cancers are urothelial (transitional) cell carcinomas (TCC), which originate in the mucous layer of surface cells of the bladder lining (transitional epithelial cells). Establishing urothelial origin of the tumor is critically important, especially when prostate cancer is also in differential diagnosis. Several studies have shown p63 to be a sensitive marker for bladder cancers and negative in prostate and kidney cancers; however, only limited studies of GATA3 and S100P have been reported in bladder, prostate and kidney cancers. The aim of this study is to examine immunohistochemical staining characteristics of GATA3, p63 and S100P antibodies on bladder TCC, and compare staining expression of GATA3 and S100P on prostate and kidney cancers.
Design: Formalin-fixed, paraffin-embedded TMA's of bladder cancers were constructed in-house and purchased commercially, and processed in the usual manner for IHC analysis. All sections were retrieved in a modified citrate buffer, in a pressure cooker at 125°C. Mouse monoclonal antibodies GATA3 and p63, and rabbit polyclonal antibody S100P, were individually optimized using a 30 minute incubation. Detection was performed using a micro-polymer and visualization with DAB chromogen.
Results: The results of GATA3, p63 and S100P in TCC are summarized in Table 1.
|Antibody||Cases||Positive||(+) %||Negative||(-) %|
|Grade 1 (11 cases)||10 (91%)||11 (100%)||11 (100%)|
|Grade 2 (37 cases)||33 (89.2%)||32 (86.5%)||33 (89.2%)|
|Grade 3 (11 cases)||10 (90.1%)||9 (81.2%)||9 (81.2%)|