The Role of MUC1, MUC2 and MUC7 in the Outcome of Superficial (Ta, T1) Urinary Bladder Cancer (SBC).
Duygu Kankaya, Saba Kiremitci, Sumer Baltaci, Zeynep Biyikli, Ozden Tulunay. Medical School of Ankara University, Turkey
Background: SBC can be cured in most cases however, it also has a very high incidence of recurrence. Therefore, there is a need for molecular markers that may identify patients at risk for recurrence and progression that would benefit from early and additional therapeutic modalities.
Design: Immunohistochemistry was performed on 99 TUR materials of SBC, using antibodies against MUC1, MUC2 and MUC7. Apical membranous (MUC1ap), superficial stratified (MUC1st), scattered (MUC1sc) and full thickness (MUC1fth) MUC1 staining patterns were evaluated and the extent of the patterns was determined on papillary (p) and inverted (i) areas of the tumors. The extent of complete membranous staining (MUC1m) was also established. Cytoplasmic (MUC2c), membranous (MUC2m), and golgi zone (MUC2gz) MUC2 staining and the extent of staining were evaluated. MUC2 expression was also evaluated for the presence of apical staining as MUC2 ap- or MUC2 ap+. The extent of cytoplasmic (MUC7c) and membranous (MUC7m) MUC7 staining was determined. The MUC7 distribution in the areas with papillary and inverted growth pattern as well as with high nuclear grade (HNG) were recorded for each tumor.
Results: Tumors with MUC1m positivity of ≥ 30% showed higher invasion rate (p=0.001) and more areas with HNG (p=0.006). MUC1pfth (p= 0.003) and MUC1ifth (p=0.024) positivity increased from Ta to invasion suspicious and T1 SBC, in spite of the decrease of MUC1pst (p= 0.009), MUC2ap (p= 0.046), MUC2m (p=0.001) staining patterns. A positive correlation was found between the extent of HNG areas and MUC1m (p=0.002), MUC7c (p=0.039) positivity whereas, a negative correlation was found with duration of tumor relapse (p=0.049). MUC2m (p=0.033), MUC2ap (p=0.009), MUC1ap (p=0.049) expressions of the tumors were correlated with prolonged duration of tumor relapse though, shortening of duration of tumor relapse with expression of MUC7c (p=0.034) was found. MUC1m expression (p=0.015) and inflammation in the tumor (p=0.025) were correlated with decreased survival rates.
Conclusions: This study demonstrates that MUCs may have a role in the assessment of the course of SBCs. MUC1fth and MUC1m expressions predict progressive course moreover, MUC1m staining, 30% or more, indicates poor survival. MUC7c expression indicates shorter duration of relapse. On the contrary, MUC2m and MUC2ap expressions identify patients with favorable prognosis.
Category: Genitourinary (including renal tumors)
Tuesday, March 1, 2011 9:30 AM
Poster Session III # 167, Tuesday Morning