Penile Squamous Cell Carcinomas: Preferential Association of Human Papillomavirus with High Histological Grade.
Alcides Chaux, Fernando Soares, Isabella da Cunha Wernecke, George J Netto, Belen Lloveras, Nubia Munoz, Silvia de Sanjose, F Xavier Bosch, Antonio L Cubilla. Johns Hopkins University, Baltimore, MD; Hospital do Cancer A. C. Camargo, São Paulo, Brazil; Instituto Catalán Oncológico, Barcelona, Spain; CIBER Epidemiologia y Salud Pública, Barcelona, Spain; Instituto de Patología e Investigación, Asunción, Paraguay
Background: Approximately 40% of penile squamous cell carcinomas (SCC) are human papillomavirus (HPV) related and there is controversy regarding the prognostic impact of HPV in this subset of tumors.
Design: SCCs (202 cases) were tested for HPV using SPF-10 PCR. They were categorized in low and high-grade and according to warty and/or basaloid (WB) features. High-grade tumors had anaplastic cells, high nuclear/cytoplasmic ratio, pleomorphism, coarse chromatin, prominent nucleoli, irregular nuclear membrane, and high mitotic/apoptotic rate.
Results: HPV was found in 63 (31%) cases. High-grade features were present in 84 (42%) of all cases. Seventy tumors (35%) showed warty and/or basaloid features. Tables 1 and 2 shown association between HPV status and histological grade in all cases, and according to the presence of WB features, respectively.
|No. cases||HPV+ (%)||HPV- (%)|
|Low-grade||118||19 (16)||99 (84)|
|High-grade||84||44 (52)||40 (48)|
|Tumors with WB features||No. Cases||HPV+ (%)||HPV- (%)||P-value|
|Low-grade||20||7 (35)||13 (65)||P=0.016|
|High-grade||50||34 (68)||16 (32)|
|Tumors without WB features||No. Cases||HPV+ (%)||HPV- (%)||P-value|
|Low-grade||98||12 (12)||86 (88)||P=0.031|
|High-grade||34||10 (29)||24 (71)|