Differentiating between Cutaneous Squamous Cell Carcinoma and Pseudoepitheliomatous Hyperplasia Utilizing QRT-PCR Analysis.
Albert Su, Seong H Ra, Xinmin Li, Scott Binder. UCLA, Los Angeles, CA
Background: Squamous cell carcinoma (SCC) is one of the most commonly diagnosed cutaneous malignancies. The diagnosis can occasionally be challenging as there are many lesions that are clinical and pathologic simulators. One of the most challenging lesions to differentiate from SCC, especially in superficial and/or limited biopsy material, is pseudoepitheliomatous hyperplasia (PEH). The goal of our study was to develop a multiplex QRT-PCR-based assay which could be conveniently used in the clinical setting to distinguish between SCC and PEH.
Design: Thirty cases of PEH and 28 cases of SCC were identified from the Tamtron database and reviewed by the authors (SR and SB). The areas of interest on the paraffin blocks were isolated and the RNA was extracted and amplified. Select primers for the following genes: S100A7, S100A8, HOXC10, KRT9 and C15orf48 were utilized for QRT-PCR analysis. The genes chosen have shown differential expression between SCC and PEH from our DNA microarray studies.
Results: Multiplex QRT-PCR was able to accurately predict SCC and PEH in 54 of 58 cases (93%). The SCC was accurately determined in 27 of 28 cases (96%) and PEH in 27 of 30 cases (90%).
Conclusions: With further refinement of the test, this QRT-PCR-based assay can be used as a molecular diagnostic tool to distinguish between SCC and PEH in paraffin embedded tissue.
Monday, February 28, 2011 9:30 AM
Poster Session I Stowell-Orbison/Surgical Pathology/Autopsy Awards Poster Session # 70, Monday Morning