Comparing MicroRNA Expression Profiles in Dermatofibrosarcoma Protuberans and Dermatofibroma.
Jonathan Lai, Xiao Zhang, Victor A Tron. Queen's University, Kingston, ON, Canada
Background: MicroRNAs (miRNAs) are small noncoding RNAs that are responsible for regulating gene expression in a variety of organisms including plants, animals, and protozoa. Expression profiles of miRNAs have been shown to differ between normal and tumor tissues, and between different tumor tissues as well. We hypothesized that miRNA expression is dysregulated in dermatofibrosarcoma protuberans (DFSP) when compared to dermatofibroma (DF).
Design: To address this, we compared miRNA expression profiles of four DFSP and four DF samples extracted from archival formalin fixed, paraffin embedded (FFPE) tissue using a miRNA array platform. This technique has been validated at our institution in a previous study (J Mol Diagn. 2008 Nov;10(6):513-9).
Results: Statistical analysis using unsupervised hierarchical clustering illustrated a clear difference in miRNA expression between the four DFSP samples when compared to the four DF samples. Supervised clustering revealed eight upregulated and nine downregulated miRNAs in DFSP relative to DF. In particular, miRNA-9 was upregulated 40 fold and miRNA-21 was downregulated 29 fold.
Conclusions: Our preliminary results suggest that miRNA expression profiles differ significantly between DFSP and DF, and may have an impact on its tumorigenesis. Further work will be done to validate our results of selected candidate miRNAs using quantitative RT-PCR and novel cloning and sequencing profiling techniques. As well, a miRNA expression profile for normal skin will be established as an additional control.
Wednesday, March 2, 2011 1:00 PM
Poster Session VI # 119, Wednesday Afternoon