Application of BRAF V600E Mutation Detection to the Bethesda Thyroid FNA Classification System at an Academic Institution.
Constantine Theoharis, Pei Hui, David Chhieng, Adebowale Adeniran. Yale University School of Medicine, New Haven, CT
Background: Thyroid Fine Needle Aspiration is the standard preoperative diagnostic procedure in the evaluation of thyroid nodules but suffers limitations. BRAF V600E positivity is associated with worsened clinical prognosis including lymph node positivity. Our purpose was to correlate BRAF mutational status based on PCR-SSCP technique in Thyroid FNA with surgical outcome and regional nodal status.
Design: 82 patients, 72% female (average age: 51 years, range 17-96) underwent BRAF PCR-SSCP testing as an adjunct test to the Bethesda Thyroid FNA Classification System at our institution beginning 2/2010. The analytical sensitivity of BRAF was previously determined to be <5%, suitable for cytological specimens.
Results: Of the 82 patients, BRAF V600E mutation was detected in 37, the remaining 45 were wild type. Pre-operative BRAF/Cytologic evaluation is summarized in Table 1.
|Cytology Dx||BRAF (+)||BRAF (-)|