[202] Reevaluation of Expression of Estrogen Receptor (ER), Progesterone Receptor (PR), GCDFP-15 and Mammaglobin in Carcinomas from Various Organs.

Haiyan Liu, Jianhiu Shi, Jeffrey W Prichard, Steven C Meschter, Fan Lin. Geisinger Medical Center, Danville, PA

Background: When working on a tumor of unknown origin, a breast primary is nearly always included in the diagnostic consideration, and a positive immunostaining profile for CK7, ER, PR, GCDFP-15 and mammaglobin supports the breast primary. However, the data in the literature are inconsistent and may not be entirely reproducible. In this study, we re-evaluated the expression of ER, PR, GCDFP-15 and mammaglobin in a large series of carcinomas from various organs using a single immunostaining system (Ventana XT).
Design: Immunohistochemical evaluation of the expression of ER, PR, GCDFP-15 and mammaglobin on 873 cases of carcinomas from various organs using tissue mircroarray sections was performed. The antibody information was summarized in Table 1.
Results: The positive staining results (%) and the total number of cases for each entity (N) are summarized in Table 2. No immunoreactivity for ER, PR, GCDFP-15, and mammaglobin was seen in lung ADC (N=55), lung SCC (N=49), papillary RCC (N=25), clear cell RCC (N=36), colonic ADC (N=38), papillary thyroid CA (N=45), follicular thyroid CA (N=36), esophageal ADC (N=30), gastric ADC (N=18), pancreatic ADC (N=70), cholangiocarcinoma (N=11), urothelial CA (N=40), prostatic ADC (N=100), adrenal cortical neoplasm (N=29) and hepatocellular C (N=18).

Table 1. Summary of the antibodies

Antibody	Vendor	Catalog No.	Clone	Dilution	AR	IT
ER	Ventana	790-4324	SP1	Predilute	CC1 Mild	40 min @ 37°C
PR	Ventana	790-2223	1E2	Predilute	CC1 Mild	24 min no heat
GCDFP-15	Cell Marque	257M-18	23A3	Predilute	CC1 Standard	32 min @ 37°C
Mammaglobin	Ventana	760-4263	31-A5	Predilute	CC1 Standard	40 min @ 42°C

AR-antigen retrieval; IT-incubation time; min-minutes; no heat-without heat treatment

Table 2. Summary of immunostaining results

Tumor	ER (%)	PR (%)	GCDFP-15 (%)	Mammaglobin (%)
Breast ductal CA	60 (102/169)	29 (40/137)	30 (53/176)	41 (72/176)
Breast lobular CA (N=48)	83	71	40	75
Endocervical ADC (N=17)	12	24	0	0
Ovarian serous CA (N=15)	80	60	0	0
Pancreatic endocrine neoplasm (PEN) (N=17)	0	53	0	0

ADC-adenocarcinoma; CA-carcinoma; RCC-renal cell carcinoma


Conclusions: Our data indicate that 1) mammaglobin and GCDFP-15 are highly specific markers for identifying a tumor of breast primary; 2) mammaglobin has a higher sensitivity than GCDFP-15 in the diagnosis of both lobular and ductal carcinomas; 3) if a gynecological primary and PEN are excluded, carcinomas from various organs are usually negative for both ER and PR.
Category: Breast

Tuesday, March 1, 2011 9:30 AM

Poster Session III # 54, Tuesday Morning