Reevaluation of Expression of Estrogen Receptor (ER), Progesterone Receptor (PR), GCDFP-15 and Mammaglobin in Carcinomas from Various Organs.
Haiyan Liu, Jianhiu Shi, Jeffrey W Prichard, Steven C Meschter, Fan Lin. Geisinger Medical Center, Danville, PA
Background: When working on a tumor of unknown origin, a breast primary is nearly always included in the diagnostic consideration, and a positive immunostaining profile for CK7, ER, PR, GCDFP-15 and mammaglobin supports the breast primary. However, the data in the literature are inconsistent and may not be entirely reproducible. In this study, we re-evaluated the expression of ER, PR, GCDFP-15 and mammaglobin in a large series of carcinomas from various organs using a single immunostaining system (Ventana XT).
Design: Immunohistochemical evaluation of the expression of ER, PR, GCDFP-15 and mammaglobin on 873 cases of carcinomas from various organs using tissue mircroarray sections was performed. The antibody information was summarized in Table 1.
Results: The positive staining results (%) and the total number of cases for each entity (N) are summarized in Table 2. No immunoreactivity for ER, PR, GCDFP-15, and mammaglobin was seen in lung ADC (N=55), lung SCC (N=49), papillary RCC (N=25), clear cell RCC (N=36), colonic ADC (N=38), papillary thyroid CA (N=45), follicular thyroid CA (N=36), esophageal ADC (N=30), gastric ADC (N=18), pancreatic ADC (N=70), cholangiocarcinoma (N=11), urothelial CA (N=40), prostatic ADC (N=100), adrenal cortical neoplasm (N=29) and hepatocellular C (N=18).
|ER||Ventana||790-4324||SP1||Predilute||CC1 Mild||40 min @ 37°C|
|PR||Ventana||790-2223||1E2||Predilute||CC1 Mild||24 min no heat|
|GCDFP-15||Cell Marque||257M-18||23A3||Predilute||CC1 Standard||32 min @ 37°C|
|Mammaglobin||Ventana||760-4263||31-A5||Predilute||CC1 Standard||40 min @ 42°C|
|Tumor||ER (%)||PR (%)||GCDFP-15 (%)||Mammaglobin (%)|
|Breast ductal CA||60 (102/169)||29 (40/137)||30 (53/176)||41 (72/176)|
|Breast lobular CA (N=48)||83||71||40||75|
|Endocervical ADC (N=17)||12||24||0||0|
|Ovarian serous CA (N=15)||80||60||0||0|
|Pancreatic endocrine neoplasm (PEN) (N=17)||0||53||0||0|