Pouring Stabilization Bodies for Paraffin Tissue Microarrays Using Agar and Top Pin Tissue Arrayers.
Ulrich F Vogel. University Hospital, Eberhard-Karls-University, Tuebingen, Baden-Wuerttemberg, Germany
Background: Paraffin tissue microarrays (PTMAs) are a well-accepted technique especially in translational pathology. Up to 2500 paraffin tissue core biopsies (PTCBs) can be installed in preformed holes in so-called recipient paraffin blocks (37 x 23 x 5 mm). To prevent the rolling and folding of PTCBs at sectioning a melting process of the filled PTMAs is recommended. However, the PTCBs may topple down during the melting process if more than one PTCB (composite PTCB) is installed in one hole of the PTMA. This problem can be solved by using so-called stabilization bodies, i.e. paraffinized agar plates (35 x 21 x 4 mm) with preformed holes. The present study should investigate whether the construction of low cost agar stabilization bodies can be easily feasible by using top pin arrayers.
Design: A boiling 2% agar solution was poured into different molds (e.g. plastic lid of a pipette tip box). Top pin arrayers (Figure 1A) were placed from above into the hot and liquid agar (Figure 1B). After solidification the pins were withdrawn resulting in agar plates with preformed recipient holes (Figure 1C). These agar plates were paraffinized in an ordinary tissue processor. The paraffinized agar plates (Figure 1D) were embedded in paraffin blocks (Figure 1E) or used as stand alones.The PTMAs were filled manually with PTCBs (Figure 1E) in a routine fashion and then completely melted (30 minutes, 60°C). After resolidification the PTMAs were cut and the sections stained according to standard procedures.
Results: It was easily feasible to pour the holes of the agar stabilization bodies using top pin arrayers. Paraffinization of these agar plates was successfully done using ordinary tissue processors. Prior to the melting process the PTCBs missed a strong contact to the surrounding paraffin (Figure 1F, top). The stabilization bodies prevented the PTCBs from toppling during the melting process. After the melting process the PTCBs firmly adhered to the surrounding paraffin and the paraffinized agar (Figure 1F, bottom).
Conclusions: The construction of low cost agar stabilization bodies is feasible using top pin arrayers. Agar stabilization bodies enhance the efficacy and quality of the PTMA technique by providing a melting procedure for composite PTCBs.
Monday, February 28, 2011 1:00 PM
Poster Session II # 285, Monday Afternoon