HPV Detection in Head and Neck Cancer Using an Automated In Situ Hybridization Approach To Decrease Turnaround Time.
Peter B Illei, William H Westra. Johns Hopkins Medical Institutions, Baltimore, MD
Background: The human papillomavirus (HPV) is driving the escalating incidence of oropharyngeal cancer, and its detection in head and neck squamous cell carcinoma is recognized as a highly relevant clinical biomarker. HPV in-situ hybridization is readily applied to routinely processed tissues facilitating its use in most diagnostic laboratories, but reproducibility is limited by technical inconsistencies. Automation could enhance standardization across diagnostic laboratories, and decrease turnaround time.
Design: An automated in situ hybridization method using the INFORM HPV III Family 16 Probe (Ventana Medical Systems, Tucson, AZ) was used to evaluate 311 head and neck squamous cell carcinomas (HNSCCs). These cases had been previously evaluated using a manual method with an HPV16 genome specific probe (Dako, Carpintera, CA) as part of clinical care (64 cases) or research (267 cases constructed onto a tissue microarray). Results for the two detection systems were compared.
Results: The automated method detected HPV in 52 of 64 (81%) clinical samples and 43 of 267 (16%) tissue array samples. There was 98% concordance between the two detection systems with only 7 discordant cases: 5 cases that were HPV-negative by the manual assay were HPV-positive with the automated assay, and 2 cases that were positive by the manual assay were negative with the automated assay.
Conclusions: HPV detection utilizing an automated in situ hybridization system can detect HPV with a very high degree of fidelity. The assay run time can be reduced to 7-8 hours from 16-24 hours. By diminishing the impact of technical inconsistencies, high throughput automation promises to decrease turnaround time for large case volumes, enhance standardization across diagnostic laboratories, and improve reproducibility among clinical trials.
Monday, February 28, 2011 1:00 PM
Poster Session II # 258, Monday Afternoon