Breast Tissue Imaging with Light Coherence Tomography: A Pilot Study.
Osnath Assayag, Adriano Burcheri, Martine Antoine, Brigitte Sigal, Claude Boccara. ESPCI ParisTech, France; Tenon Hospital, Paris, France; Curie Institute, Paris, France; LLTech, Paris, France
Background: Breast cancer is the most common cancer and the second leading cause of death for women in western countries. Despite advances in early detection, breast imaging, and increased diagnosis of small tumors (cT1), up to 36% of breast-conserving surgery patients require subsequent excisions to achieve disease-free margins.The peroperatory evaluation of margins during surgery is time consuming, and the research for new techniques is justified. This pilot study aims to evaluate Light Coherence Tomography (Light-CT) as a tool for optical biopsies and imaging of benign and malignant human breast tissues.
Design: A Light CT scanner based on Full Field Optical Coherence Tomography (FFOCT) has been used to image human breast tissues. The light CT scanner has a<2µm axial resolution and <2µm transverse resolution. 40 breast specimens have been imaged from at least 10 different patients covering healthy tissues from post and pre-menopausic women, lobular and ductal carcinoma, and sentinel nodes, after or without fixation. The time required and safety of a Light-CT imaging procedure have also been evaluated.
Results: Good structural correspondence is observed between Light-CT images and histological sections.
Characteristic features such as ducts, lobules, vessels, adipous, fibrous and necrotic tissue can be identified from Light-CT images and are recognized on normal and malignant tissue. Morphometric data such as the size of lobules and ducts and thickness of the parietal wall of the ducts and vessels as characteristics of these structures related to the presence of elastic tissue, can provide an additional diagnostic information. 9x9mm Light-CT images have been obtained in 2,5 minutes. Light-CT technology is safe since histology has been possible on 100% of the breast tissue samples.
Conclusions: We have demonstrated that the light CT technology is a fast and safe tool that can be used under clinical conditions. The Light CT images allow to recognize breast tissue structures and pathologies by comparison with traditional histology.
Monday, February 28, 2011 1:00 PM
Poster Session II # 280, Monday Afternoon