microRNA Profiling in Adipocytic Neoplasms.
Will A Ahrens, Nury M Steuerwald, Josh C Patt, Jeffrey S Kneisl, Herbert L Bonkovsky. Carolinas Medical Center, Charlotte, NC
Background: MicroRNAs (miRNAs) are a class of small noncoding RNAs. They have been shown to promote or suppress tumorigenesis in human cancers by altering cancer-related gene expression. Subtype-specific expression patterns are emerging in various types of sarcoma. In this study, miRNA expression profiling was performed in 18 adipocytic tumors.
Design: Snap-frozen tumor was collected from 7 well-differentiated liposarcomas (WDLS), 4 myxoid liposarcomas (MLS), 2 lipoblastomas (LB) and 5 lipomas (LP). Non-neoplastic adipose was collected in 6 cases as control. RNA was isolated using Qiagen miRNeasy. RNA was Poly A tailed and ligated to biotinylated signal molecules using the FlashTag Biotin RNA labeling Kit (Genisphere). An Enzyme Linked Oligosorbent Assay QC assay was performed to verify labeling prior to hybridization to GeneChip miRNA Arrays (Affymetrix, Santa Clara, CA). Hybridization, washing, staining and scanning was performed using Affymetrix GeneChip system instruments. Affymetrix GeneChip Operating Software version 1.4 was used to analyze microarray image data and to compute intensity values. Partek Genomic Suite (Partek Inc., St. Louis, MI, USA) was used to compute fold changes and perform statistical analyses.
Results: Tumor subtype principal component analysis is shown in figure 1.
The 4 MLS grouped closely together. >10 fold up-regulation vs control was observed for hsa-miRs 486-5p/451/29a and 150. >10 fold down regulation was observed for hsa-miRs 891a/181a/181c/493 and 1247. LB showed >10 fold up-regulation vs control for hsa-miRs 486-5p/451/1246 and 182. >10 fold down regulation was observed for has-miRs 433/493/409-5p/382/431/485-5p and 376c. 1 LB showed overlap of 14 individual miRs with the MLS cluster. WDLS and LP grouped with control cases. The p value for all fold changes was <0.05.
Conclusions: This data supports that notion that miRNA profiling can be used to sub-classify adipocytic neoplasms with histologic overlap. Importantly, identification of specific miRNAs and their targets may reveal tumor markers with prognostic value as well as targets for treatment intervention in sarcoma.
Category: Bone & Soft Tissue
Monday, February 28, 2011 1:00 PM
Poster Session II # 23, Monday Afternoon