Proteomic Analysis of the Lung in Rats with Hypobaric Hypoxia-Induced Pulmonary Hypertension.
Yuichiro Ohata, Sho Ogata, Kuniaki Nakanishi, Sadayuki Hiroi, Susumu Tominaga, Toshiaki Kawai. National Defense Medical College, Tokorozawa, Saitama, Japan
Background: The experimental pulmonary hypertension that develops in hypobaric hypoxia is characterized by structural remodeling of the lung.
Design: Using proteomics -- perhaps the most powerful way to uncover unknown remodeling proteins involved in the enhancement of cardiovascular performance -- we analyzed lungs from 150 male Wistar rats housed in a chamber at the equivalent of the 5500m altitude level for up to 21 days.
Results: After 14 days' exposure to hypobaric hypoxia, pulmonary arterial pressure (PAP) was significantly increased. In lung tissue, about 140 matching protein spots were found among 8 groups (divided by hypobaric periods) by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) (pH4.5-pH6.5, 30kDa-100kDa). In hypobaric rats, three spots were increased two fold or more (vs. control rats) using differential in-gel electrophoresis (DIGE). The increased proteins were identified, by matrix-assisted laser desorption ionization time of flight (MALDI-TOF), as one isoform of protein disulfide isomerase associated 3 (PDIA3), and two isoforms of heat shock protein 70 (HSP70). These two proteins were confirmed by Western blotting analyses using 2D-PAGE.
Conclusions: Conceivably, PDIA3 and HSP70 may play roles in modulating the structural remodeling that occurs in the lung due to pulmonary hypertension in hypobaric hypoxia.
Wednesday, March 2, 2011 9:30 AM
Poster Session V # 272, Wednesday Morning