microRNAs as a Tool for the Subtyping of Non-Small Cell Lung Carcinoma (NSCLC).
Maria L Cabanas, Alfons Navarro, Ramon Marrades, Marc Campayo, Nuria Vinolas, Tania Diaz, Mariano Monzo, Jose Ramirez. IDIBAPS, CIBERES, Hospital Clínic, Barcelona, Spain; Universidad de Barcelona, Spain; IDIBAPS, Hospital Clínic, Barcelona, Spain
Background: The classification of NSCLC has become very important in the last few years, because of the specific treatment and clinical side effects implied. There is a large number of miRNAs that can be of use in making the diagnosis of different types of carcinoma. The aim of this study is to establish the utility of different microRNAs in accurately subclassifying malignant lung tumors.
Design: We have studied 146 cases of lung tumors, 72 histologically diagnosed as squamous cell carcinoma and 74 as adenocarcinoma. In this series we studied 79 frozen tissue and 67 formalin-fixed, paraffin-embedded tissue samples. All these cases were analysed by Real Time-PCR for: miR-21, miR-205 and miR-218. Data were analyzed with SPSS 15.0 by using Discriminant analysis and T-Test.
Results: According to our results, 83% of the cases that were originally diagnosed as adenocarcinoma or squamous cell carcinoma had a characteristic expression profile when considering miR-205, miR-21 and miR-218. The level of expression of miR-205 was higher in adenocarcinoma than in squamous cell carcinoma, being this difference statistically significant (p<0.001). The comparison between both tumors did not show any significance when assessing the level of expression of miR-21 and miR-218. In both series of frozen and paraffin-embedded tissue samples, the results were similar and therefore significant.
Conclusions: We conclude that miR205 may become an important aid to ascertain the pathological diagnosis of the main subtypes of NSCLC. These results should encourage further studies to confirm this data, as well as suggest the possible application to serological studies for early diagnosis in patients with lung cancer. Supported by: FIS 08/0135
Monday, February 28, 2011 9:30 AM
Poster Session I Stowell-Orbison/Surgical Pathology/Autopsy Awards Poster Session # 223, Monday Morning