The Roles of BDNF and SDF-1 Expression Levels and Polymorphisms in the Development of Intraventricular Hemorrhage in the Premature Infant Population.
Yue Wu, Sandra Canosa, Qi Li, Vladmir Glinskii, Irina Buhimuschi, Joseph Madri. Yale University School of Medicine, New Haven
Background: Low birth weight preterm infants suffer from perinatal hypoxic-ischemic encephalopathy. Increased BDNF and SDF-1 levels were correlated with the survival /responsiveness of neuron stem cells. The BDNF Val66Met polymorphism was demonstrated in neurological impairments. SDF-1 G801A polymorphism is involved in tumor invasion. These findings have prompted this cord blood study, with a goal to provide novel biomarkers in predicting the risk for severe/persistent neurodevelopmental handicaps.
Design: 1. Cord blood samples were collected from preterm infants with weight <1250 gram, and divided into three groups: intraventricular hemorrhage only (IVH), early-onset sepsis status only (ENOS) and controls without intraventricular hemorrhage or sepsis (NO IVH). 2. ELISA determination of cord blood BDNF. 3. BDNF and SDF-1 polymorphism determination using DNA loci of interest amplification and RFLP analysis.
Results: 1. BDNF level was 113.4 pg/mg in IVH group, significantly less than that of No IVH control group (151.5 pg/mg, p<0.01).
2. BDNF Val/Met (GA) polymorphism was detected in 25% IVH, and in 16% ENOS; while Met/Met (AA) was shown in 12.5% control group. 3. SDF-1 G801A polymorphism was detected in 50% control group, markedly higher than that of IVH group (11%), and EONS group (0%).
Conclusions: 1. BDNF-1 cord blood level was significantly decreased and Val66Met polymorphism was slightly increased in preterm infants with IVH. 2. SDF-1 G801A polymorphism, a genotype known to promote tumor cell metastasis, was markedly reduced in preterm infants with IVH or ENOS, which might account for their decreased neurogenesis. The increased incidence of this polymorphism in the control infant brain will presumably foster the stimulation of neurogenesis following hypoxic insult.
Wednesday, March 2, 2011 9:30 AM
Poster Session V # 244, Wednesday Morning