PIT-1, and ERα Transcription Factors, and PTTG-1 Gene Expression in Pituitary Adenomas.
Alma Ortiz-Plata, Monica Gutierrez de la Pena, Aurora Sanchez-Garcia, Ivan Perez-Neri, Martha L Tena Suck, Daniel Rembao-Bojorquez. National Institute of Neurology and Neurosurgery, Mexico City, DF, Mexico
Background: Pituitary adenomas (ADHs) are diagnosed as benign tumors even though they can be aggressive. In the pituitary development transcriptions factors play an important role in determining cell phenotypes and proliferation. Overexpression of transcription factors has been detected in ADHs suggesting their role in the formation of pituitary tumors. Objective: The aim was to evaluate the secretory activity, transcription factors expression and proliferative markers in ADHs.
Design: In 99 pituitary adenomas immunohistochemistry against PCNA, Ki-67 antigen, Pit-1, ERα, and pituitary hormones (prolactin, growth hormone, FSH, LH, TSH, ACTH) was made. Correlation analysis among hormonal activity, cellular proliferation, and transcription factors expression was done.
Results: ERα expression correlate with GH, ACTH hormones, and plurihormonal adenomas (p=0.047, p=0.008, p=0.042 respectively); Pit-1 had correlation with prolactin hormone and non-secretor ADHs; high Ki-67 expression correlate with Pit-1 (p= 0.019) and ERα (p= 0.017), and PCNA correlate with Pit-1 (r2=0.145, p=0.001), and was high in secretor than in non-secretor ADHs (p=0.034). PTTG-1 shows correlation with plurihormonal tumors in general (p=0.014), with FSH and TSH in particular (p= 0.056; p=0.053), and with non-secretor (p=0.040). Also PTTG-1 expression is associated with ERα expression (p=0.000) and PCNA expression (p=0.028).
Conclusions: Pit-1 and ERα are involved in proliferation inducing the cyclin D1 expression; Pit-1 over expression has been founded in GH, Prl and TSH adenomas. Is strongest predictor of prolactinoma development, ERα and Pit-1 transcription factors expression shows that it can be useful to distinguish the hormonal activity of the ADHs overall in plurihormonal ones in which its diagnostic is difficult, and its correlation with the proliferative markers could help to give an idea of their biological behaviour.
Monday, February 28, 2011 9:30 AM
Poster Session I Stowell-Orbison/Surgical Pathology/Autopsy Awards Poster Session # 213, Monday Morning