Applications of Optical Coherence Tomography (OCT) in Neuropathology.
Tim-Rasmus Kiehl, Beau A Standish, Victor X Yang. University Health Network, Toronto, ON, Canada; Ryerson University, Toronto, ON, Canada
Background: Optical coherence tomography (OCT) is an imaging modality that has the potential for significant clinical impact in image-guided biopsy. The very high, near histological resolution (1-10 micron) and vascular imaging provided by OCT promises to improve the accuracy in the targeting of core biopsies. Only very limited investigations have been reported to date for applications in pathology, and none in neuropathology.
Design: In this study, we first sought to evaluate potential applications in the neurosurgical intraoperative consultation. Next, we investigated the effect of different fixatives on subsequent OCT imaging quality. Lastly, the ability of OCT to image brain autopsy material was assessed.
Results: For intraoperative frozen sections, we found that parameters such as cellularity and necrosis could be reliably detected, potentially improving tissue sampling in the future. Formalin fixation resulted in inferior resolution while fixation in an ethanol-based fixative preserved the OCT-visibility of many tissue structures. On unfixed brain autopsy tissue, OCT could reliably demonstrate blood vessels and even individual neurons.
Conclusions: OCT may represent a rapid way to extract information from tissue without requiring fixation, processing or sectioning. No tissue is permanently used up or altered in the process. In the future, OCT may have additional applications in pathology, such as in 3D reconstruction and tissue banking.
Tuesday, March 1, 2011 1:00 PM
Poster Session IV # 245, Tuesday Afternoon