[1557] Utility of pVHL, Maspin, S100P and IMP3 in Diagnosis of Carcinomas from Various Organs.

Fan Lin, Hanlin L Wang, Myra Wilkerson, Haiyan Liu. Geisinger Medical Center, Danville, PA; Cedars-Sinai Medical Center, Los Angeles, CA

Background: Our studies demonstrated that pVHL, S100P, maspin, and IMP3 were the useful panel of antibodies in confirming a diagnosis of pancreatobiliary adenocarcinoma (AJSP 2008;32:78; Hum Pathol 2010;40:324), and pVHL was a sensitive and specific marker for identifying clear cell carcinoma of the kidney, uterus and ovary (AJCP 2008;129:592). In this study, we investigate the potential utility of these markers in the diagnosis of carcinomas from other organs.
Design: Immunohistochemical evaluation of the expression of pVHL, maspin, S100P and IMP3 was performed on a total of 900 cases of carcinomas from various organs on tissue microarray sections.
Results: The positive immunostaining results (%) and a total number of cases for each entity (N) are summarized in the Table 1. Twenty-two cases of intratubular germ cell neoplasia (ITGCN) were all positive for IMP3.

Table 1. Summary of the immunostaining results

Tumor	pVHL (%)	Maspin (%)	S100P (%)	IMP3 (%)
Lung ADC (N=54)	4	51	4	65
PRCC (N=20)	100	0	0	0
CRCC (N=40)	95	0	0	10
Colonic ADC (N=38)	16	90	55	50
Thyroid papillary CA (N=45)	0	9	0	4
Thyroid follicular CA (N=36)	0	8	0	0
Esophageal ADC (N=30)	13	100	73	57
Gastric ADC (N=18)	0	67	67	56
Pancreatic ADC (N=70)	0	100	96	90
Gallbladder ADC (N=23)	0	100	74	78
Urothelial CA (N=40)	0	65	68	35
Intrahepatic cholangiocarcinoma (ICC) (N=11)	73	27	9	55
Breast ductal CA (N=176)	0	26	24	6
Lobular CA (N=83)	4	31	55	6
Endocervical ADC (N=17)	6	65	47	53
Ovarian serous CA (N=15)	0	27	0	53
Prostatic ADC (N=100)	0	2	1	0
HCC (N=18)	17	0	11	17
Seminoma (N=30)	0	0	0	100
Embryonal CA (N=24)	0	21	0	100
Yolk sac tumor (N=12)	0	17	0	100

ADC-adenocarcinoma; RCC-renal cell CA; CA-carcinoma; HCC-Hepatocellular CA


Conclusions: Our data demonstrate that 1) IMP3 is a sensitive marker for seminomas, non-seminomatous tumors and ITGCN; 2) maspin can be used as a useful marker for esophageal ADC; 3) pVHL can be positive in a small percentage of carcinomas of the colon, esophagus and liver; 4) IMP3 is usually negative in both ductal and lobular CA of the breast and can potentially serve as a marker for differential diagnosis; 5) pVHL is expressed in a high percentage of ICC, which can be useful in differentiating it from other biliary tract ADC if confirmed in a large series of cases.
Category: Liver & Pancreas

Tuesday, March 1, 2011 1:00 PM

Poster Session IV # 210, Tuesday Afternoon