[1557] Utility of pVHL, Maspin, S100P and IMP3 in Diagnosis of Carcinomas from Various Organs.

Fan Lin, Hanlin L Wang, Myra Wilkerson, Haiyan Liu. Geisinger Medical Center, Danville, PA; Cedars-Sinai Medical Center, Los Angeles, CA

Background: Our studies demonstrated that pVHL, S100P, maspin, and IMP3 were the useful panel of antibodies in confirming a diagnosis of pancreatobiliary adenocarcinoma (AJSP 2008;32:78; Hum Pathol 2010;40:324), and pVHL was a sensitive and specific marker for identifying clear cell carcinoma of the kidney, uterus and ovary (AJCP 2008;129:592). In this study, we investigate the potential utility of these markers in the diagnosis of carcinomas from other organs.
Design: Immunohistochemical evaluation of the expression of pVHL, maspin, S100P and IMP3 was performed on a total of 900 cases of carcinomas from various organs on tissue microarray sections.
Results: The positive immunostaining results (%) and a total number of cases for each entity (N) are summarized in the Table 1. Twenty-two cases of intratubular germ cell neoplasia (ITGCN) were all positive for IMP3.

Table 1. Summary of the immunostaining results
TumorpVHL (%)Maspin (%)S100P (%)IMP3 (%)
Lung ADC (N=54)451465
PRCC (N=20)100000
CRCC (N=40)950010
Colonic ADC (N=38)16905550
Thyroid papillary CA (N=45)0904
Thyroid follicular CA (N=36)0800
Esophageal ADC (N=30)131007357
Gastric ADC (N=18)0676756
Pancreatic ADC (N=70)01009690
Gallbladder ADC (N=23)01007478
Urothelial CA (N=40)0656835
Intrahepatic cholangiocarcinoma (ICC) (N=11)7327955
Breast ductal CA (N=176)026246
Lobular CA (N=83)431556
Endocervical ADC (N=17)6654753
Ovarian serous CA (N=15)027053
Prostatic ADC (N=100)0210
HCC (N=18)1701117
Seminoma (N=30)000100
Embryonal CA (N=24)0210100
Yolk sac tumor (N=12)0170100
ADC-adenocarcinoma; RCC-renal cell CA; CA-carcinoma; HCC-Hepatocellular CA

Conclusions: Our data demonstrate that 1) IMP3 is a sensitive marker for seminomas, non-seminomatous tumors and ITGCN; 2) maspin can be used as a useful marker for esophageal ADC; 3) pVHL can be positive in a small percentage of carcinomas of the colon, esophagus and liver; 4) IMP3 is usually negative in both ductal and lobular CA of the breast and can potentially serve as a marker for differential diagnosis; 5) pVHL is expressed in a high percentage of ICC, which can be useful in differentiating it from other biliary tract ADC if confirmed in a large series of cases.
Category: Liver & Pancreas

Tuesday, March 1, 2011 1:00 PM

Poster Session IV # 210, Tuesday Afternoon


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