A Comparison of the Immunophenotype of Hepatocellular Carcinoma and Non-Lesional Hepatocytes When Analyzed with Next-Generation Markers.
Stephen M Lagana, Marcela Salamao, Fei Bao, Roger K Moreira, Jay H Lefkowitch, Helen Remotti. Columbia University Medical Center, New York, NY
Background: Many novel immunohistochemical stains have recently been introduced to help distinguish hepatocellular carcinomas (HCC) from non-malignant hepatocytes. Among these are Glypican-3 (GPC-3), heat shock protein 70 (HSP-70), and glutamine synthetase (GS). The role of Glucose-transporter-1 (GLUT-1) has been controversial in HCC. In addition, a recent study demonstrated Arginase-1 (Arg-1) to be a more sensitive and specific marker of hepatocellular differentiation than hepatocyte paraffin antigen (HepPar1) in HCC. We undertook a comparative study of these markers in HCC and non-lesional hepatocytes.
Design: Tissue microarrays were constructed from 47 cases (17 high grade, 30 low grade) including both HCC and non-lesional (mostly cirrhotic) liver. Staining was performed for the above markers. A cut-off of 5% was set for positivity.
|Sensitivity (%)||High grade (17)||53||65||94||6||88||100|
|Low grade (30)||47||50||93||0||87||97|
|Average percentage of cells staining in positive cases||High grade||49||49||72||5||72||90|