The Expression of Apoptotic Related Proteins and Apoptosis in Human Renal Tissues of Class II and IV Lupus Nephritis.
Jing Zhang, Jihong Cui, YaQing Zhang, Qing Qiao. Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China; Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China
Background: Apoptosis is involved in glomerular injuries leading to glomerulonephritis. The role of renal cell apoptosis in the pathogenesis and progress of human lupus nephritis (LN) is still controversial. Furthermore, in different types of LN renal tissues, the expression of apoptotic related proteins, such as FasL, Bax and caspase-3, is still unknown. We therefore investigated these apoptotic related proteins and apoptosis index (AI) in human renal tissues of class II and IV LN.
Design: The expressions of FasL, Bax and caspase-3 were assessed in forty-two cases of human LN renal tissues (twenty cases of class II, twenty-two cases of class IV) and ten cases of human normal renal tissues by immunocytochemistry. The terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was used to assess AI.
Results: In class II and IV LN renal tissues, the increased expressions of FasL, Bax, caspase-3, and AI were detected in glomerular cells, tubular epithelial cells compared with controls (P<0.05). The expression of Bax, caspase-3 and AI in glomerular cells of class IV LN was significantly higher than class II LN (P<0.05). However, there was no difference in FasL expression between class II LN and class IV LN (P>0.05).
Conclusions: Apoptosis might be induced to LN pathogenesis by FasL, Bax and caspase-3. There might be other pathways except Fas/FasL signaling to initiate apoptosis during LN progress.
Category: Kidney (does not include tumors)
Wednesday, March 2, 2011 1:00 PM
Poster Session VI # 245, Wednesday Afternoon