Telemicroscopy for the Evaluation of Donor Kidney Biopsies.
Evita Sadimin, Wenjin Chen, Adena Osband, William Reitsma, David Laskow, Jaworski Joseph, Billie Fyfe. UMDNJ – Robert Wood Johnson Medical School, New Brunswick, NJ; New Jersey Sharing Network, New Providence
Background: Pathologists play a vital role in the evaluation of kidney donor biopsies, largely related to accurate measurement of % global glomerulosclerosis (%GG). We have previously shown lack of correlation for %GG using light microscopy (LM) with manual slide manipulation. This study examines the correlation of %GG measurements using telemicroscopy (TM) enhanced with Computer Assisted Microscopy (CAM) software.
Design: Thirty-seven kidney donor biopsies with prior LM analysis performed at regional transplant centers and at our institution were studied with TM. The real time robotic TM incorporates an Olympus BX51 microscope with a robotic stage controlled by CAM software. The software provides the user an overview of the specimen that is used to select the region of interest (ROI). The ROI is overlaid with a grid map. Each grid cell corresponds to one non-overlapping screen of view. The user sets the desired objective and activates the program to advance from grid cell to grid cell. Analyzed cells are highlighted by a color change. The specimens were evaluated for %GG and total number of glomeruli using a 10x grid with full support for panning, zooming and focusing. The correlation coefficient is calculated using MedCalc.
Results: The correlation coefficient for %GG and total glomeruli measured by TM is significantly higher than LM.