Classification of Diffuse Large B-Cell Lymphoma with a New Algorithm and Evaluation of GCET2 in the Classification System.
Zenggang Pan, Min Li, Omar Hameed, Wing C Chan, Zifen Gao. University of Alabama at Birmingham; Peking University Health Science Center, Beijing, China; University of Nebraska Med. Center, Omaha
Background: GCET1 has been incorporated in a new algorithm using 5 antibodies (GCET1, CD10, BCL6, MUM1 and FOXP1) to classify diffuse large B-cell lymphoma (DLBCL) into germinal center B-cell-like (GCB) and activated B-cell-like (ABC) subgroups (Clin Cancer Res. 2009: 5291-5303), which is significantly more accurate than the previous Hans' algorithm (Blood. 2004, 103: 275-282). However, a small proportion of DLBCLs remain difficult to be subclassified. We studied a series of DLBCLs with the new algorithm and evaluated the potential role of another GC B-cell marker, GCET2, in classification of DLBCL.
Design: Four tissue microarrays containing 92 DLBCL cases were stained using monoclonal antibodies against GCET1, GCET2, BCL6, CD10, MUM1 and FOXP1. These DLBCL cases were classified as GCB or ABC subtypes based on the new algorithm. The sensitivity and specificity of each GC B-cell markers were calculated.
Results: The 92 DLBCL cases were classified into 59 ABC-DLBCL (27 nodal and 32 extranodal) and 33 GCB-DLBCL (15 nodal and 18 extranodal). The ABC to GCB subtypes had a highest ratio in the extranodal, non-gastrointestinal locations (18:6).
|GCET1||12||47||24||9||73% (24/33)||80% (47/59)|
|GCET2||21||29||22||8||73% (22/30)||58% (29/50)|
|BCL6||22||34||25||8||76% (25/33)||61% (34/56)|
|CD10||0||59||17||16||52% (17/33)||100% (59/59)|