CD57+ Natural Killer Cells Are Associated with Better Overall Survival in DLBCL Patients.
Jeong Hyeon Lee, Yoon Jin Kwak, Chul Hwan Kim, Insun Kim. Korea University Anam Hospital, Seoul, Korea; College of Medicine, Korea University, Seoul, Korea
Background: The malignant tumor cells may evoke the innate and the adaptive immune system, and various types of immune cells are supposed to be involved these immune reaction. It has been studied that tumor infiltrating lymphocytes, macrophages or natural killer (NK) cells are correlated with good prognosis of the patients. In this study, the prognostic significance of tumor infiltrating helper/cytotoxic T cells, tumor associated macrophages (TAM), and natural killer (NK) cells was evaluated in the patients with diffuse large B cell lymphoma (DLBCL).
Design: Tissue microarray block was constructed from the paraffin block of 78 cases of DLBCL between 2001 and 2009. All cases were reviewed and confirmed that the histologic and immunophenotypic criteria for the WHO classification of lymphoid neoplasms for DLBCL were fulfilled. Clinical and follow-up data were obtained by reviewing the medical records. Clinical parameters included age, sex, performance status(PS), Ann Arbor clinical stage, serum LDH levels, number of extranodal sites, international prognostic index (IPI) score, degree of response to chemotherapy, and survival rate. Antibody for CD57, CD68, CD4 and CD8 was used identification of NK cell, TAM, helper and cytotoxic T cells, respectively. For enumeration of infiltrating cells, one image from representative area of each case was created with Olympus DP70 camera, connected to Olympus equipped with an UPlanApo 40 x objectives (Olympus, Japan). An area of 0.88mm2 was investigated for each case.
Results: The patients with high CD57+ NK cells (>30cells/0.88mm2) showed significantly higher overall survival rate than the patients with low CD57+ NK cells (p=0.040). However, there was no statistically significant difference between the number of CD57+ NK cells and other prognostic parameters. The extents of CD68+ TAMs and CD4+ or CD8+ T cells did not have any significant correlation with prognostic factors in the patients with DLBCL.
Conclusions: The evaluation of tumor infiltrating CD57+ NK cells is recommended in diagnosis of DLBCL, as a prognostic indicator of the patient.
Wednesday, March 2, 2011 1:00 PM
Poster Session VI # 213, Wednesday Afternoon