[1188] Histologic Features of Oral Squamous Cell Carcinoma Invasive Front Correlate with Genetic Markers of Survival and/or Metastasis.

Jennifer R LaPointe, Melissa P Upton, David R Doody, John Houck, Pawadee Lohavanichbutr, Eduardo Mendez, Neal Futran, Chu Chen. University of Washington, Seattle; Fred Hutchinson Cancer Research Center, Seattle

Background: Histologic features of oral squamous cell carcinoma (OSCC) previously associated with biologic outcome include nuclear pleomorphism, keratinization, cell organization, and inflammation at the invasive front. We (Oralchip study, NIH/NCI 095419) previously used fresh tumor tissue and Affymetrix genechips to identify gene expression markers associated with survival and/or metastasis. Expression levels of these markers have not been previously correlated with histologic features.
Design: Two pathologists blinded to the OralChip results independently examined H&E slides from 84 OSCC patients for 21 histologic features. Discrepant results were re-examined jointly. Histologic results were correlated with marker expression values from corresponding fresh tumor samples.
Results:

Histologic features significantly associated with gene marker expression.

Markers of:	Histologic feature (p-value ≤)
Decreased Survival
LAMC2	Prominent desmoplasia (0.002)
	Eosinophils present (0.021)
	Myxoid stroma (0.039)
	Increased nuclear pleomorphism (0.044)
OSMR	Myxoid stroma (0.005)
SERPINE1	Dissociated/non-cohesive growth (<0.001)
	Myxoid stroma (0.001)
	Perineural invasion (0.001)
	Basaloid features absent (0.016)
	Prominent desmoplasia (0.037)
OASL	Absence of basaloid features (0.005)
	Increased keratinization (0.024)
SLC16A1	Increased keratinization (0.002)
	Prominent squamous eddies (0.004)
	Myxoid stroma (0.008)
	Eosinophils present (0.014)
	Granulomatous response absent (0.031)
	Prominent desmoplasia (0.042)
Metastasis
MYO5A	Increased keratinization (<0.001)
	Basaloid features absent(0.001)
	Prominent squamous eddies (0.004)
RNF145	Decreased keratinization (0.001)
	Histiocyte response absent (0.010)
	Basaloid features present (0.037)
FBOX32	Perineural invasion (0.008)
	Eosinophils absent (0.047)
CTONG2002744	Prominent squamous eddies (0.022)

Conclusions: We identified histologic features at the OSCC invasive front that are significantly associated with genetic markers of decreased survival and/or risk of metastasis. Further investigation to explore these associations is warranted.
Category: Head & Neck

Monday, February 28, 2011 9:30 AM

Poster Session I Stowell-Orbison/Surgical Pathology/Autopsy Awards Poster Session # 150, Monday Morning