Clusterin Overexpression Promotes Cell Proliferation and Inhibits Apoptosis in Primary Ovarian Cancer.
Richard A Owings, Guofen Yang, Yu Liu, Chun-Yang Fan. Univ Arkansas for Med Sciences, Little Rock; The First Affiliated Hospital, Sun Yat-Sen Univ Med Sciences, Guangzhou, China; Guangdong Specialty Hospital of Fertility, Guangzhou, China; Central Arkansas Veterans' Healthcare System and UAMS, Little Rock
Background: Clusterin, a multifunctional glycoprotein, is ubiquitously produced in mammalian tissues. While clusterin has been shown to play significant roles in many aspects of human tumor biology, such as cell proliferation, apoptosis, chemoresistance and angiogenesis, the relationship of clusterin expression with cell proliferation and apoptosis in ovarian cancer has not been studied.
Design: Immunohistochemical (IHC) staining for clusterin, Ki-67 and Bcl-2 were performed on a Tissue Microarray (TMA) containing 181 primary ovarian epithelial cancer. These 181 tumors consisted of 119 serous carcinoma, 23 mucinous carcinoma and 39 other types of carcinoma (endometroid, mixed mullerian, clear cell, Brenner, and undifferentiated). A total of 158 cases were available for evaluation for all 3 markers. The levels of protein expression for these 3 genes were scored based on staining intensity and percentage of immunopositive cells and were correlated with one another.
Results: Among these 158 cases, clusterin, Ki-67 and Bcl-2 were overexpressed in 95 (60%), 60 (37%) and 26 (16%) of the cases respectively. Among 95 tumors that overexpressed the clusterin, Ki-67 and Bcl-2 were increased in expression in 40 (43%) and 18 (18%) cases respectively. By contrast, among 63 tumors without significant clusterin expression, overexpression of Ki-67 and Bcl-2 were seen in only 20 (31%) and 8 (12%) of the cases.
Conclusions: Overexpression of clusterin in epithelial ovarian cancer appears to be correlated with increased cancer cell proliferation and decreased cancer cell apoptosis, consistent with the established role of clusterin as an oncogene in the biology of ovarian cancer.
Category: Gynecologic & Obstetrics
Tuesday, March 1, 2011 1:00 PM
Poster Session IV # 114, Tuesday Afternoon