Biomarkers of Invasion in Cervical Squamous Cell Carcinoma.
Ana Felix, Fernanda Silva, Sergio Dias. Instituto Português de Oncologia e Faculdade de Ciências Médicas, Lisboa, Portugal; Instituto Português de Oncologia, Lisboa, Portugal
Background: The capacity of neoplastic cells to invade and metastasize depends on different factors, namely on the ability to establish new complex interactions of cellular adhesion molecules and the extracellular matrix (ECM) components. Laminin γ2 chain (lamγ2) plays an important role during tumor invasion and integrins are primary receptors mediating ECM remodeling. Our aim was to identify biomarkers of invasion in cervical squamous cell carcinomas.
Design: Laminin5 (Lamγ2) α2; α3; α5 and αV integrins immunoexpression was evaluated in a tissue microarray (TMA) constructed with representative regions (formalin fixed and paraffin embedded tumor samples) of 81 cervical squamous cell carcinomas (11 being in situ and the remaining 70 were invasive) and appropriate controls. Staining was evaluated both in cells and in the ECM.
Results: Lamγ2 was present in the ECM (basement membrane) in all in situ carcinomas (CINIII) and also in the cytoplasm of very few cells, in two cases. In contrast, intracytoplasmatic lamγ2 was present in 59 cases of invasive SCC (see table 1). This difference was significant (p<0.0001). Regarding integrins, we found that α5 integrin was overexpressed in invasive tumor. This finding was also significantly correlated with invasion (p=0.0043). In invasive tumors α5 and αv (cell membrane) integrins were significantly correlated with the presence of intra-cytoplasmatic lamγ2 expression (p= 0.0009 and p=0.0053, respectively).
|ECM||100%||36%||not found||not found||9%|
|CIN III||11||Cell cytoplasm||18%||9%||73%||0%||55%|
|Cell membrane||not found||91%||73%||0%||55%|
|ECM||86%||21%||not found||not found||29%|
|Cell membrane||not found||86%||63%||40%||71%|