Expression of RHO Family Proteins in Ovarian Endometriosis.
Belen Canet, Inigo Espinosa, Jaime Prat. Hospital de la Santa Creu i Sant Pau, Autonomous University of Barcelona, Barcelona, Spain
Background: Recently, we found that RHO family proteins may contribute to explain the singular biologic behaviour of ovarian clear cell carcinomas, particularly their detection at early stage. Currently, it is believed that most clear cell and endometrioid carcinomas arise from ovarian endometriosis. However, little is known about the expression of RHO family proteins in ovarian endometriosis.
Design: We analyzed by Real Time PCR RHOA, RHOC, CDC42 and three ARHGDIs (Rho GDI dissociation inhibitors) genes in 20 solitary endometriotic cysts, 12 endometriotic cysts with clear cell carcinoma, and 4 endometriotic cysts with endometrioid carcinoma. Immunoreaction for CDC42 was also investigated in 22 endometriotic cysts (10 solitary cysts, 8 cysts associated with clear cell carcinoma, and 4 cysts with endometrioid carcinoma). Only the non-tumor components were evaluated for RNA and protein expression.
Results: Solitary endometriotic cysts (not-associated with carcinoma) showed higher expression of CDC42 than endometriotic cysts with cancer (P=0.047). Furthermore, endometriotic cysts with endometrioid carcinoma had higher levels of ARHGDIB mRNA than endometriotic cysts with clear cell carcinoma (P=0.009). CDC42 was exclusively expressed by the macrophages. The proportion of solitary endometriotic cysts with high levels of CDC42-positive macrophages was higher than that of endometriotic cysts with cancer (6/10; 60% vs. 3/10; 30%).
Conclusions: Our results indicated that CDC42-positive macrophages and ARHGDIB may play an important role in early endometrioid and clear cell carcinogenesis.
Category: Gynecologic & Obstetrics
Tuesday, March 1, 2011 1:00 PM
Poster Session IV # 108, Tuesday Afternoon