[1005] Tumor Associated Macrophages and Correlation with Prognostic Factors in Endometrial Adenocarcinomas.

Summer L Bohman, Erica C Dun, Evelyn A Reynolds, Jie Yi, Fritz Wieser, Robert N Taylor, Krisztina Hanley. Emory University, Atlanta, GA

Background: The pathomechanism and significance of tumor associated macrophages (TAMS) is not fully understood. While some thought TAMS are a result of a host immune response, recent studies suggest that TAMS play an important role in tumorgenesis and angiogenesis. The aim of our study is to evaluate if the number and location of TAMS have any correlation with various prognostic findings in endometrial adenocarcinomas (EAC).
Design: Forty cases of various EAC were selected. Tumor type, grade, stage, presence of lymphovascular invasion (LVI), and clinical outcome were recorded. Immunohistochemical staining for CD68 (DakoCytomation, Carpinteria, CA) was performed on each tumor. The number of TAMS was counted, averaged over 10 high powered fields, and stratified into those associated with tumor epithelium or stroma. The data was then analyzed for correlation with various prognostic factors.
Results: There were 22 endometroid, and 18 serous or clear cell carcinomas included. The number of TAMS in the epithelium and stroma was correlated with tumor characteristics (Table 1).

Table 1: Correlation of Tumor Associated Macrophages and Tumor Characteristics in Endometrial Adenocarcinomas
 Subjects (N=40)Epithelial TAMs: Meanp ValueStromal TAMs: Meanp Value
Histology  0.127 0.609
Endometroid228.1 17.1 
Serous157.9 15.1 
Clear Cell32.7 14.9 
FIGO Stage  0.870 0.403
I156.6 13.2 
II58.7 15.3 
III148.4 21.0 
IV67.5 13.2 
FIGO Grade  0.867 0.133
1107.4 12.6 
297.4 23.4 
3217.9 14.8 
Invasion  0.925 0.577
Absent66.5 12.7 
Present347.8 16.8 
LN Mets  0.829 0.964
Unknown115.8 15.2 
Absent158.0 17.1 
Present148.6 16.0 
Lymph-Vascular Invasion  0.416 0.357
Absent196.5 18.5 
Present218.7 14.1 
Alive/Dead  0.988 0.479
Alive287.5 15.5 
Dead128.0 17.7 

Conclusions: Although our study did not show statistically significant correlation between TAMS and any of the prognostic factors in EAC, we concluded the following: There is an overall greater number of TAMS seen in tumor related stroma than in the epithelium. More TAMS (epithelial and stromal) are seen in tumors showing myoinvasion and/or lymph node metastasis. Finally, there are a slightly increased number of epithelial associated TAMS seen in tumors with LVI than those without.
Category: Gynecologic & Obstetrics

Wednesday, March 2, 2011 1:00 PM

Poster Session VI # 170, Wednesday Afternoon


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