[967] The Expression of Vitamin D Associated Markers in High Grade Prostatic Intraepithelial Neoplasia (HGPIN)

S Sasturkar, A Blutreich, TW He, M Nagar, J Small, L Chiriboga, R Hayes, J Melamed, J Ahn. NYU School of Medicine, New York, NY

Background: There has been increasing evidence that vitamin D may participate in early prostate cancer carcinogenesis. We have examined tissue protein expression of vitamin D activating (CYP27A1) and deactivating (CYP24) enzymes of 1,25-dihydroxy vitamin D, an active vitamin D metabolite and the vitamin D receptor (VDR) in high grade prostate intraepithelial neoplasia (HGPIN) compared to adjacent normal prostate tissue.
Design: HGPIN tissue microarrays were constructed from radical prostatectomy specimens (n=46 cases) to include foci of HGPIN (4 cores/case) and of non-neoplastic peripheral zone. Immunohistochemical studies using commercially available antibodies against CYP24 (Goat Polyclonal C-18; Santa Cruz), CYP-27A1(Goat Polyclonal P-17; Santa Cruz) and VDR (Rabbit Polyclonal C-20; Santa Cruz) were performed on an automated immunostainer (Benchmark; Ventana Medical Systems, Tucson, AZ). Tissue microarray stained slides were scored manually using an intensity + proportion score (range: 0-8). The mean expression level difference between HGPIN and non-neoplastic prostate tissue was tested by t-test.
Results: The cohort studied included 46 male, mean age 61 (range 46-74), mean Gleason score 6.6 (range 6-7), tumor stage pT2-pT3. The expression of these markers was as follows:

Table 1 showing Marker Expression
MarkerHGPINNon neoplastic Prostate tissuesignificance by t-test
CYP-27A15.76.1P < 0.05
VDR6.16.4P < 0.05

We found that gene expression levels in CYP27A1 and VDR in HGPIN are significantly lower than non-neoplastic prostate tissue.
Conclusions: These results suggest that a decrease in the vitamin D activating enzyme, CYP-27A1 and the Vitamin D nuclear receptor, VDR may play a role in the decreased Vitamin D metabolism in early prostate cancer development.
Category: Genitourinary (including renal tumors)

Wednesday, March 24, 2010 9:30 AM

Poster Session V # 101, Wednesday Morning


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