Cell Surface Peptidases CD13 and CD10 in Renal Cell Neoplasms
S Gobbo, M Brunelli, D Segala, E Munari, M Chilosi, F Menestrina, G Martignoni. University of Verona, Verona, Italy
Background: Renal cell tumors originating from the proximal tubule, clear cell renal cell carcinoma and papillary renal cell carcinoma, show a worse behaviour than chromophobe renal cell carcinoma and renal oncocytoma which display differentiation toward distal nephron. CD10 has been described to be a specific marker for clear cell and papillary renal cell carcinoma even if its immunoexpression has been observed also in a consistent portion of chromophobe renal cell carcinomas and in some renal oncocytomas. CD13 is another surface peptidase and recently a monoclonal antibodies have been reported to be applicable to human paraffin-embedded specimens. The current study compares CD13 and CD10 protein expression by immunohistochemical and western blot analyses in a large series of renal cell tumors and evaluates their capability to distinguish “proximal renal cell tumors” from “distal renal cell tumors”.
Design: We analyzed the immunoexpression of CD10 and CD13 in 166 renal cell neoplasms including 79 clear cell renal cell carcinomas, 34 papillary renal cell carcinomas, 25 chromophobe renal cell carcinomas and 24 renal oncocytomas. We performed western blot analysis to confirm the expression of these proteins.
Results: Both CD13 and CD10 immunoreactions showed a membranous staining pattern. CD10 was observed in 94% of clear cell renal cell carcinomas, in 43% of papillary renal cell carcinomas, in 40% of chromophobe renal cell carcinomas and in 15% of renal oncocytomas. CD13 was expressed in 78% of clear cell renal cell carcinomas, and in 76% papillary renal cell carcinomas whereas neither chromophobe renal cell carcinomas nor renal oncocytomas showed any immunoreaction for CD13. Western blot analysis confirmed the presence of CD10 in chromophobe renal cell carcinoma and in renal oncocytoma and the protein expression of CD13 in clear cell renal cell carcinoma and papillary renal cell carcinoma.
Conclusions: We concluded that CD13 is more sensitive and specific than CD10 to distinguish “proximal renal cell tumors”, clear cell and papillary renal cell carcinomas, from “distal renal cell tumors” such as chromophobe renal cell carcinomas and renal oncocytomas.
Category: Genitourinary (including renal tumors)
Wednesday, March 24, 2010 9:30 AM
Poster Session V # 111, Wednesday Morning