FoxM1 Immunohistochemical Expression Profiles in Prostatic Adenocarcinoma
TL Bartholow, AV Parwani. University of Pittsburgh School of Medicine, Pittsburgh, PA
Background: The transcription factor Forkhead Box M1, or FoxM1, has been shown to be involved in cell proliferation. Abnormal FoxM1 expression patterns have been tied to cervical cancer, breast cancer, lung cancer, and pancreatic cancer. In transgenic mouse models, its expression has been shown to accelerate tumor development and proliferation. In this study, we examined the immunostaining intensites of FoxM1 in prostatic adenocarcinoma.
Design: 9 cases of normal donor prostates, 12 cases of benign prostatic hyperplasia (BPH), 12 cases of prostatic intraepithelial neoplasia (PIN), 49 cases of primary prostatic adenocarcinoma, and 13 cases of normal tissue adjacent to prostatic adenocarcinoma were used to construct tissue microarrays and immunostained for FoxM1. An automated imaging analysis system was used to quantitate intensity scores. Additional analysis was performed comparing prostatic adenocarcinoma specimens by grade and stage, along with a comparison of primary versus metastatic prostatic adenocarcinomas.
Results: PIN, BPH, and primary prostate adenocarcinoma groups had the highest absolute staining scores as compared to normal donor prostates and the normal tissue adjacent to adenocarcinoma. Significant differences were noted between PIN and normal tissue adjacent to prostatic adenocarcinoma (p = 0.004), BPH and normal tissue adjacent to prostatic adenocarcinoma (p = 0.008), and primary prostatic adenocarcinoma and normal tissue adjacent to prostatic adenocarcinoma (p = 0.047). Interestingly, no differences were seen when prostatic adenocarcinoma specimens were compared by grade or when metastatic prostatic adenocarcinoma was compared to primary prostatic adenocarcinoma. Stage 3 prostatic adenocarcinoma had a statistically significant increased staining intensity when compared to Stage 4 (p = 0.008).
Conclusions: FoxM1 has critical functions in tumor progression but the mechanisms by which FoxM1 is involved in these processes are not clearly understood. To our knowledge, ours is one of the first studies that focuses on evaluating the FoxM1 expression profiles of a large number of cases of prostatic carcinoma . In this study, no consistent statistical differences were seen between normal donor tissue, BPH, and PIN in comparison to primary prostatic adenocarcinoma. An interesting finding from the current study was that there was a stastical difference noted in the staining intensities between select tumor stages. This warrants further investigation in future targeted studies and may have important implications in prostate neoplastic progression.
Category: Genitourinary (including renal tumors)
Wednesday, March 24, 2010 9:30 AM
Poster Session V # 104, Wednesday Morning