Prevalence and Genotype Identification of HPV Infection in Penile vs. Vulvar Carcinomas
E Poblet, A Pascual-Martin, M Pariente-Martin, J Chiarri-Rodrigo, R Vera-Beron, JM Godinez. Hospital General Universitario de Albacete, Albacete, Spain; Hospital Ramón y Cajal, Madrid, Spain; Institut Catala d´Oncologi, L´Hospitalet, Barcelona, Spain
Background: Human papillomavirus (HPV) has been detected in cases of penile and vulvar carcinomas, and it appears that certain types of HPV are an important risk factor for the development of these tumors. The aim of this study was to compare the prevalence of HPV and the implicated genotypes in penile and vulvar carcinomas. In order to make that comparison possible, we studied patients from the same regional area and we used the same methodology in all cases. This study will provide valuable information to determine the possible correlation that carcinomas of male and female external genitalia may present.
Design: Samples from 49 patients with penile carcinoma and from 37 patients with vulvar carcinoma were selected. Formalin-fixed, and paraffin-embedded specimens were collected from the archives of the Pathology Department of our Hospital. DNA was extracted from the paraffin blocks, and PCR technique was performed in conditions that prevented contamination. All the cases of this study were tested with two different sets of consensus primers (GP5+/GP6+ and My09/My11) in order to improve the sensitivity of the technique. Appropriate positive and negative controls were run in parallel. To determine the implicated genotypes positive cases were sequenced.
Results: The overall prevalence of HPV was found to be 77.5% in penile carcinoma and 30.3% in vulvar carcinoma. From the HPV positive patients with penile carcinoma, genotype 16 was detected in thirty-two cases (84,2%), genotype 18 in four (10,5%), and in two subjects, results were inconclusive (5.2%). Besides HPV16 or 18, there were no other genotypes detected in any of the cases of penile carcinoma; however, HPV6 and HPV11 were found in benign penile lesions that were run in parallel. Genotype characterization of vulvar carcinoma cases showed that HPV16 was present in two patients (6,1%), HPV18 in four patients (12,1%), HPV33 in three patients (9,1%), and HPV 35 in one patient (3,0%).
Conclusions: This study shows that the HPV prevalence detected in vulvar carcinomas is lower than that detected in penile carcinomas, and that the HPV genotypes identified in these tumors are markedly different. The results show low HPV correlation between carcinomas of male and female external genitalia, and indicate that HPV participate differently in the pathogenesis of these tumors.
Wednesday, March 24, 2010 1:00 PM
Poster Session VI # 91, Wednesday Afternoon