Galectin-3 and BRAF Mutation in Cases of Cytologically Suspicious of Papillary Carcinoma
DK Kim, HK Kim, JY Kwak, MJ Kim, HJ Jung, JS Koo, BJ Lim, CK Jung, SW Hong. Yonsei University College of Medicine, Seoul, Korea; Catholic University St. Mary's Hospital, Seoul, Korea
Background: Fine needle aspiration, known as the most accurate and cost-effective manner, still have some chances of indeterminate cases which are pauci-cellular and showing minor nuclear atypia, but most of are suspicious of papillary thyroid carcinoma (PTC). BRAF mutation was found about half of the PTC and galectin-3 as expressed by malignant tumor, helping us to differentiate malignancy from benign lesion.
Design: Subjected cases included 44 cases previously diagnosed as suspicious of PTC with cytologic examination which were confirmed with PTC after tissue examination and 4 benign controls. The subjected cases were analyzed the galectin-3 expression by immunohistochemical staining and the BRAF mutation by PCR-RFLP (Polymerase chain reaction-restriction fragment length polymorphism) with new restriction enzyme.
Results: All 44 cases of PTC and 2 out of 4 benign controls revealed galectin 3 expression. BRAF mutation was found in 9 cases of PTC only.
|Category(n)||Galectin-3 (+)||Galectin-3 (-)|
|Galectin-3 and BRAFV600E||100||100||100||100||100|
|Galectin-3 or BRAFV600E||100||55.5||87||84.6||100|