Subclinical Human Papillomavirus Infections Are Common among Women under Thirty Years of Age
MF Evans, CSC Adamson, TL St. John, CA Weaver, K Cooper, DL Weaver. University of Vermont, Burlington, VT; Fletcher Allen Health Care, Burlington, VT
Background: The aim of this study was to conduct a thorough survey of the range and frequency of human papillomavirus (HPV) infections in young women with normal cervical cytology. These data are required for understanding HPV natural history and the wider context of clinical HPV screening, and for substantiating the ages at which exposure to high-risk (hr) HPV infections occurs.
Design: Cervical cytology samples diagnosed as negative for an intraepithelial lesion or malignancy (NILM) were collected from 214 women aged 13-19, 166 women aged 20-24, and 186 women aged 25-29 years. Purified DNA extracts were initially screened for HPV by PCR using GP5+/6+ and PGMY09/11 primers. Samples that tested HPV negative by these assays were also screened using FAP59/64 primers. HPV genotypes were identified by dot blot hybridization (GP5+/6+ assay) and/or by cycle sequencing (PGMY09/11 and FAP59/64 assays).
Results: Table 1 summarizes the findings.
|Age Range in Years||13-19||20-24||25-29|
|Number of Patients||214||166||186|
|Any HPV Type Positive||85¹(39.7%)||88² (53.0%)||58³ (31.2%)|
|High-Risk (hr) HPV∗ Positive||49¹′ (22.9%)||57²′ (34.3%)||32³′ (17.2%)|
|HPV16 or 18 Positive||31 (14.5%)||38 (22.9%)||20 (10.6%)|