Epithelial-Mesenchymal Transition in Breast Carcinomas
H Jeong, Y Ryu, J An, Y Lee, A Kim. Korea University Guro Hospital, Seoul, Korea, Seoul, Korea; Samsung Medical Center, Seoul, Kyrgyzstan
Background: Epithelial-mesenchymal transition (EMT) is characterized in loss of epithelial nature and acquisition of mesenchymal nature. EMT is proposed as important step in tumor invasion and metastasis. However EMT may be transient and cannot be identifiable in human tumor. Recent studies suggest that EMT occurs in specific genetic context, basal phenotype of breast carcinomas.
Design: To assess a role of EMT in human mammary carcinogenesis, we performed immunohistochemical studies for EMT markers(vimentin, SMA, E-cadherin, N-cadherin, CK19 and Cam 5.2)using tissue microarray sections.
Results: Total 492 cases were evaluated in this study and classified into hormonal receptor positive (HR) type, Her-2 type, and triple negative (TN) type using immunohistochemistry and in situ hybridization. Among the 102 cases of TN type breast cancer, 24.5%, 13.7%, and 9.8% expressed vimentin, N-cadherin, and smooth muscle actin. However, among the 221 cases of HR type breast cancer, 4.1%, 5.9%, and 0.4% expressed vimentin, N-cadherin, and smooth muscle actin, respectively. Decreased expression of epithelial type marker-E-cadherin, CK19, and CAM 5.2-was observed in 16.7%, 45.1%, and 60.8% of TN type breast cancer. Meanwhile, 11.7%, 6.8%, and 3.2% of HR type breast cancer showed decreased expression of E-cadherin, CK19 and CAM5.2, respectively. Loss of epithelial nature and acquisition of mesenchymal characteristic was related to the TN type of breast cancer (p=0.000). It also showed significant association with high histological grade (p=0.000). However, there was no difference in disease free survival of patients whether the EMT was observed or not.
Conclusions: We showed that the EMT is related to the molecular expression profile of breast cancer, particularly, TN type, and high histological grade.
Wednesday, March 24, 2010 1:00 PM
Poster Session VI # 2, Wednesday Afternoon