[1955] A Simple and Very Flexible Top Pin Tissue Arrayer Using Loose Pins

UF Vogel. University Hospital, Eberhard-Karls-University, Tuebingen, Baden-Wuerttemberg, Germany

Background: Paraffin tissue microarrays (PTMAs) are constructed by injecting paraffin tissue core biopsies (PTCBs) into preformed holes of a recipient block. These holes can be punched (Kononen), drilled (Vogel) or poured (Mengel). Devices to pour the holes normally consist of a modified steel mold with steel pins at the bottom (Figure 1A; TMA builder, LabVision). So, the size of the recipient block and the arrangement and the number of the holes are determined by the steel mold. To make the system more flexible I designed a device which uses ordinary steel molds of different size, a spacer plate with loose steel pins and a perforated plate. In contrast to the modified steel molds the steel pins are placed from above into the ordinary steel molds and are loose.
Design: 375 holes (diameter: 1.05 mm; distance of the holes: 0.3 mm) were drilled into a spacer plate (POM-C; Figure 1 B,C; S spacer plate) and a perforated plate (nickel silver; Figure 1B,C; P perforated plate) within an area of 32 x 20 mm². Depending on the desired number of holes in the future PTMA up to 375 steel pins (diameter: 1.0 mm; length: 30 mm) were put into the holes of the spacer plate. The steel pins had a 1.2 mm swelling at one end to prevent gliding through the holes. The spacer plate equipped with the desired number and arrangement of steel pins, the perforated plate, a plastic cassette (Figure 1C; C cassette) and an ordinary steel mold (tissue-tek; Figure 1C; M mold) were put together like a sandwich and filled with liquid paraffin. Optionally, a cover plate (Figure 1BC; CP cover plate) was added to prevent the loose steel pins to fall out of the spacer plate. After solidification, the sandwich was disassembled to release the recipient block (Figure 1D). Paraffin tissue core biopsies were manually injected into the holes of the recipient block. The PTMAs were cut and the sections were stained according to routine procedures.


Results: PTMAs with different size and with different numbers and different arrangements of the PTCBs were successfully constructed.
Conclusions: With the novel device consisting of a spacer plate, a perforated plate and loose steel pins it is possible to pour recipient blocks using ordinary steel molds of different size making the system very flexible.
Category: Techniques

Monday, March 22, 2010 1:00 PM

Poster Session II # 260, Monday Afternoon

 

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