Genotype-Phenotype Correlation in Lung Adenocarcinoma
VE Klepeis, EG Mark, D Dias-Santagata, AJ Iafrate, M Mino-Kenudson. Massachusetts General Hospital & Harvard Medical School, Boston
Background: With the advancement of personalized treatment for non-small cell lung cancer, especially adenocarcinoma (ADA), identification of molecular targets in an efficient fashion is of the utmost importance in therapeutic decision making. However, there have been controversial reports on using histologic features to predict the presence of some mutations.
Design: Seventy-four ADAs, surgically resected or excised and submitted for molecular testing, were classified based on WHO criteria as ADA with bronchioloalveolar (mucinous or non-mucinous), papillary, acinar and/or solid patterns. Of those, 110 described mutations of 13 genes were studied using mutiplex PCR in 58 tumors (33 positive for mutation, 25 negative for known mutation). Point mutations of EGFR or KRAS were found by direct sequencing in the remaining 16 cases. The association between dominant histologic features and mutations were evaluated.
Results: While the majority of cases studied displayed a mixed histologic pattern, a dominant pattern was always evident.
|All patterns||BAC, non-mucinous||BAC,mucinous||Papillary/Micropapillary||Acinar||Solid||Others|