Detection of FOXO1A, SYT and DDIT3 Gene Break Aparts by Fluorescence In-Situ Hybridization in Various Carcinomas of Epithelial Origin
S Narendra, J Tull, S Zhang. SUNY Upstate Medical University, Syracuse, NY
Background: FOXO1A, SYT and DDIT3 gene break-aparts associated with chromosomal translocations can be identified in the majority of alveolar rhabdomyosarcomas, synovial sarcomas and myxoid liposarcomas, respectively, and have been widely used as specific molecular markers in diagnosis of these neoplasms. However, the frequency and specificity of these genetic markers as well as their clinical utility in carcinomas of epithelial origin have not been studied.
Design: Tissue samples ranged from small biopsies to wide resections, diagnosed from 2000 to 2007. Commercial probes of fluorescence in situ hybridization (FISH) for FOXO1A, SYT and DDIT3 gene break aparts were applied in 43, 42 and 31 cases respectively of paraffin embedded tissues of different carcinomas. The carcinomas included the tissue origins of breast, ovary, lung, thyroid, urinary bladder, cervix, pancreas, parotid, adrenal, gallbladder, kidney, skin, colon, testes and larynx.
Results: With appropriate positive and internal controls, there were no FOXO1A, SYT or DDIT3 gene breaks identified in 43, 42 and 31 cases of carcinomas, respectively.
Conclusions: FOXO1A, SYT and DDIT3 gene break aparts are extremely rare events, if any, in various carcinomas of epithelial origin, and remain specific biomakers for alveolar rhabdomyosarcoma, synovial sarcoma and myxoid liposarcoma respectively.
Wednesday, March 24, 2010 9:30 AM
Poster Session V # 231, Wednesday Morning