Expression of the Extracellular Matrix Protein Periostin in Liver Tumours and Bile Duct Carcinomas
MO Riener, FR Fritzsche, A Soltermann, H Moch, GO Kristiansen. University Hospital, Erlangen, Germany; University Hospital, Zurich, Switzerland
Background: Epithelial-mesenchymal transition (EMT) is a process by which an epithelial cell gains features of a mesenchymal cell. EMT enables the cell to migrate more easily which is an important event during malignant tumor progression and metastasis. The periostin protein has been found to be involved in EMT. In different tumor types its expression was noted in tumor cells and/or stromal cells and correlated with increased tumor aggressiveness and reduced overall-survival. Here, we studied the relevance of Periostin, known to be involved in EMT, in hepatocellular and bile duct cancer.
Design: Immunohistochemical Periostin expression was semiquantitatively analyzed in normal liver tissue (n=20), hepatocellular carcinoma (HCC; n=91), liver-cell adenoma (n=9), focal nodular hyperplasia (n=13) and bile duct carcinomas (BDC; n=116) using tissue microarrarys.
Results: Normal bile ducts, gall bladder epithelium and hepatocytes showed weak cytoplasmic Periostin expression. In HCC, there was strong epithelial Periostin expression in 19/91 (20.9%) and strong stromal Periostin expression in 10/91 cases (11%). Epithelial expression in tumour cells was significantly associated with a higher tumour grade (p<0.05) and HBV infection (p=0.007). Importantly, there was no strong Periostin expression in benign liver tumors. Strong stromal Periostin expression was detected in 78/116 (67.2%) BDC and strong epithelial expression in 39/116 (33.6%) BDC. pT stage, differentiation grade and proliferation rate in primary BDC were independent of Periostin expression. Epithelial Periostin expression was associated with reduced overall-survival in univariate and multivariate analysis.
Conclusions: The EMT protein Periostin is expressed in the stroma and epithelium of a subset of BDC and HCC. Epithelial Periostin expression is a marker for malignant transformation of hepatocytes and a novel prognostic marker in BDC.
Category: Liver & Pancreas
Tuesday, March 23, 2010 9:30 AM
Poster Session III # 231, Tuesday Morning