Expression of Ezrin in Neoplastic and Nonneoplastic Liver Lesions
W Li, D Tan, P Zhang. 1The University of Texas Medical School at Houston, Houston, TX; University of Texas M.D.Anderson Cancer Canter, Houston, TX; University of Texas M.D. Anderson Cancer Canter, Houston, TX
Background: Ezrin is a cytoskeleton-associated protein involved in involved in cell adhesion to the extracellular matrix, cell–cell communication, signal transduction, and apoptosis. Ezrin has been implicated in cancer metastasis and progression in a variety of neoplasms. This study aims to evaluate immunohistochemical expression of the Ezrin in neoplastic and nonneoplastic liver lesions, and assess its value in the diagnosis of liver tumors.
Design: Formalin-fixed paraffin-embedded tissue sections of 73 hepatocellular carcinoma (HCC) (17 well, 37 moderately and 19 poorly differentiated), 15 hepatocellular adenoma, 63 cirrhotic nodules and 19 normal liver tissues were immunostained for Ezrin using standard avidin-biotin techniques. The level of Ezrin expression was categorized into four grades: 0 (negative), 1+ (weak), 2+ (moderate), or 3+ (strong) based on intensity of staining. The expression of Ezrin was further assessed using two scales: high expression (2+ or 3+) and low expression (0 or 1+).
Results: Expression of Ezrin is significantly enhanced in HCC tissue compared with non-HCC tissue. High expression of Ezrin was found in 86 % of HCC tissue including 89 % of well-differentiated HCC. In contrast, only 10 % of hepatocellular adenoma, 17 % of cirrhotic nodules and 5 % of normal liver tissues showed high expression for Ezrin. The differences in Ezrin expression between well-differentiated HCC and hepatocellular adenoma or cirrhotic nodules was significant (P < 0.001). No significant correlation was found between Ezrin expression and HCC grade.
Conclusions: The expression of Ezrin is highly enhanced in HCC. Ezrin staining may serve as a useful marker for the differential diagnosis of well-differentiated HCC from hepatocellular adenoma. Increased Ezrin expression suggests that Ezrin may play a role in HCC development and progression. The molecular studies of Ezrin in HCC are currently under investigation.
Category: Liver & Pancreas
Tuesday, March 23, 2010 9:30 AM
Poster Session III # 224, Tuesday Morning