Impact on Cell Proliferation of Proteins of the AKT/mTOR and MAPK Pathways in Human Pancreatic Adenocarcinoma
A Handra-Luca, P Hammel, V Rebours, A Sauvanet, A Martin, R Fagard, JF Flejou, J Belghiti, P Bedossa, P Ruszniewski, A Couvelard. APHP Univ Paris 13, Bondy, France; APHP Univ Paris 7, Clichy, France; APHP Univ Paris 13, Bobigny, France; APHP Universite Paris 13, Bobigny, France; APHP Univ Paris 6, Paris, France
Background: The mitogen activated protein kinase (MAPK) and AKT pathways are main signaling pathways involved in cell proliferation, survival and chemoresistance in pancreatic ductal adenocarcinoma (PDAC) carcinogenesis.
Design: We aimed to analyze the expression of the main proteins of the MAPK (ERK1/2, P38, MKK4) and AKT (AKT, mTOR) pathways with respect to cell proliferation in PDAC. The expression of phosphorylated mTOR (p-mTOR), p-AKT, p-ERK1/2, p-P38; of AKT2, MKK4 and of Ki67 was determined by immunohistochemistry in 99 PDAC. Medians of the protein expression score (based on intensity and percentage of stained tumor cells) and of the percentage of Ki67 stained nuclei, were used to classify tumors. Statistical analysis used the Fisher's and Kendall's rank correlation tests.
Results: The expression of the 3 MAPK was coordinated.
|Kendall's Tau||p value||95% Confidence Interval|