[1329] Natural Killer Cell (NK) Subsets and NK-Like T-Cell Populations in Benign and Malignant B-Cell Proliferations Vary Based on Clinicopathologic Features

SE Gibson, SH Swerdlow, RE Felgar. University of Pittsburgh School of Medicine, Pittsburgh, PA

Background: NK cells include less mature (CD56+, CD16-) and more mature (CD56 dimmer+, CD16+) subsets. Whereas it is known that the more mature subset is more frequently found in peripheral blood vs. lymph node, the relative distribution of these subsets, as well as NK cells in general, have received little attention in B-cell lymphoproliferations.
Design: The proportions of CD3-, 7+, 56+, 16/57+ vs. 16/57- NK subsets and CD3+, 56+ ± 16/57+ NK-like T-cells were determined by flow cytometry in 150 B-NHL and 89 non-neoplastic tissue biopsies. Results were correlated with the clinicopathologic findings.
Results: A higher percentage of NK cells was found in benign spleens vs. other non-neoplastic tissues (p<0.001) and in splenic-based B-NHL vs. B-NHL at other sites (p<0.01). More mature CD56+, 16/57+ NK cells were relatively more numerous in benign spleens (p<0.001) and non-splenic B-NHL (p<0.01) vs. non-splenic, non-neoplastic tissue as well as in diffuse large B-cell lymphoma (DLBCL) vs. other B-NHL (p<0.001) and in follicular lymphoma (FL) with an intermediate/high vs. low FLIPI score (n=17, p=0.04). No stage-related differences were found in DLBCL (n=19, Ann Arbor) or CLL/SLL (n=16, Rai). A higher proportion of NK-like T-cells was seen in DLBCL vs. other B-NHL (p=0.001).

NK subsets and NK-like T-cells in non-neoplastic tissue and B-NHL (mean ± std dev)
No.%CD56+, 16/57- NK% CD56+, 16/57+ NK% Total NK% Total NK-like T
All non-neoplastic8975.0 ± 19.425.0 ± 19.43.5 ± 4.84.0 ± 4.1
Non-neoplastic LN3280.2 ± 10.419.8 ± 10.40.9 ± 0.52.0 ± 1.3
Non-neoplastic spleen2963.1 ± 22.436.9 ± 22.48.1 ± 5.85.7 ± 4.3
Non-neoplastic extranodal2881.7 ± 18.318.3 ± 18.31.8 ± 2.34.7 ± 5.0
All B-NHL15070.1 ± 24.029.9 ± 24.01.2 ± 2.36.1 ± 7.8
LN-based B-NHL9671.5 ± 24.428.5 ± 24.40.8 ± 1.25.7 ± 8.9
Splenic-based B-NHL1265.2 ± 26.134.8 ± 26.15.9 ± 5.58.5 ± 5.4
Extranodal-based B-NHL4268.3 ± 22.931.7 ± 22.90.7 ± 0.76.4 ± 5.4
DLBCL3556.7 ± 23.343.3 ± 23.31.0 ± 0.912.2 ± 13.3
FL3875.2 ± 19.924.8 ± 19.90.9 ± 1.25.6 ± 4.2
CLL/SLL2969.8 ± 26.930.2 ± 26.91.6 ± 4.22.5 ± 2.3



Conclusions: The proportions of NK, NK subsets, and NK-like T-cells vary based on tissue site in benign settings and based on the type of B-NHL. A higher relative proportion of mature CD56+, 16/57+ NK cells are found in spleens, in more aggressive B-NHL and in FL of higher stage.
Category: Hematopathology

Monday, March 22, 2010 1:00 PM

Poster Session II # 153, Monday Afternoon

 

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