Granulocyte, Monocyte and Blast Immunophenotype Abnormalities in Acute Myeloid Leukemia with Myelodysplasia-Related Changes
SP Ayar, S Ravula, JM Polski. University of South Alabama, Mobile
Background: Flow cytometric immunophenotyping (FCI) is a useful and widely utilized technique for diagnosis of acute myeloid leukemia (AML). Routine FCI of AML is usually limited to blast analysis. However, compelling evidence exists that granulocyte and monocyte immunophenotype abnormalities exist in myelodysplastic syndromes or myeloproliferative neoplasms. Little literature exists regarding granulocyte and monocyte immunophenotype abnormalities in AML. We hypothesized that granulocyte and monocyte immunophenotype abnormalities are common in AML and especially AML with myelodysplasia-related changes (AMLMRC).
Design: Bone marrow or peripheral blood specimens from 48 cases of AML and 22 cases of control specimens were analyzed by FCI. The AML cases were classified as AMLMRC according to the most recent WHO Classification criteria. Granulocyte, monocyte, and blast immunophenotype abnormalities were compared between cases of AML vs. controls and AMLMRC vs. AML without myelodysplasia. Chi-square test was used for statistical analysis.
Results: Out of 48 cases of AML, 22 were AMLMRC, 16 were AML without myelodysplasia and 10 lacked information for complete evaluation. Granulocyte, monocyte, and blast abnormalities were common in AML compared to controls and the differences were statistically significant.
|Type of abnormality||AML||Controls||Significance|
|All of the above||11/48||0/22||p=0.036|
|Type of abnormality||AMLMRC||AML without MRC||Significance|
|All of the above||9/22||1/16||p=0.043|