[1282] Granulocyte, Monocyte and Blast Immunophenotype Abnormalities in Acute Myeloid Leukemia with Myelodysplasia-Related Changes

SP Ayar, S Ravula, JM Polski. University of South Alabama, Mobile

Background: Flow cytometric immunophenotyping (FCI) is a useful and widely utilized technique for diagnosis of acute myeloid leukemia (AML). Routine FCI of AML is usually limited to blast analysis. However, compelling evidence exists that granulocyte and monocyte immunophenotype abnormalities exist in myelodysplastic syndromes or myeloproliferative neoplasms. Little literature exists regarding granulocyte and monocyte immunophenotype abnormalities in AML. We hypothesized that granulocyte and monocyte immunophenotype abnormalities are common in AML and especially AML with myelodysplasia-related changes (AMLMRC).
Design: Bone marrow or peripheral blood specimens from 48 cases of AML and 22 cases of control specimens were analyzed by FCI. The AML cases were classified as AMLMRC according to the most recent WHO Classification criteria. Granulocyte, monocyte, and blast immunophenotype abnormalities were compared between cases of AML vs. controls and AMLMRC vs. AML without myelodysplasia. Chi-square test was used for statistical analysis.
Results: Out of 48 cases of AML, 22 were AMLMRC, 16 were AML without myelodysplasia and 10 lacked information for complete evaluation. Granulocyte, monocyte, and blast abnormalities were common in AML compared to controls and the differences were statistically significant.

Type of abnormalityAMLControlsSignificance
Granulocyte22/484/22p=0.050
Monocyte23/482/22p=0.004
Blast44/480/22p<0.001
All of the above11/480/22p=0.036
None0/4818/22p<0.001


The abnormalities were more common in AMLMRC cases compared to AML without myelodysplasia.

Type of abnormalityAMLMRCAML without MRCSignificance
Granulocyte14/225/16p=0.049
Monocyte12/226/16p=0.298
Blast22/2213/16p=0.132
All of the above9/221/16p=0.043
None0/220/16N/A


The difference reached statistical significance for abnormalities of granulocytes and abnormalities in all cells of interest. From the numerous individual abnormalities, only CD25 expression in blasts was significantly more prevalent in AMLMRC in this study (p=0.005).
Conclusions: The results confirmed our previous results showing granulocyte and monocyte abnormalities in AML. Association of some of the abnormalities with AMLMRC was statistically significant. We conclude that detection of granulocyte, monocyte, and blast immunophenotype abnormalities can contribute to the diagnosis of AMLMRC.
Category: Hematopathology

Monday, March 22, 2010 9:30 AM

Poster Session I Stowell-Orbison/Surgical Pathology/Autopsy Awards Poster Session # 177, Monday Morning

 

Close Window