Increased Fibroblast Density and Syndecan-1 Expression in the Stroma of Lip Cancer Samples
ML Spencer, P Zapata, S Aguayo, AJ Mejia, A Martinez, IG Rojas. University of Concepcion, Concepcion, Chile
Background: Squamous Cell Carcinoma (SCC) of the lip is a type of oral cancer that affects the lip vermillion. Its main etiologic factor is ultraviolet sunlight, and the premalignant and malignant forms of lip SCC have shown increased prevalence in countries located near the Antarctic ozone hole, such as Chile and Australia. It has been demonstrated that the stroma surrounding several neoplasias plays a crucial role in cancer progression. Syndecan-1 is a cell-surface heparan sulfate proteoglycan which is involved in cell proliferation, adhesion and migration. During carcinogenesis, syndecan-1 expression is reduced in cancer cells, whereas its expression is increased in stromal cells, specifically in activated fibroblasts. Therefore the objectives of this study were to determine fibroblast density in the stroma of lip SCC and normal lip samples and to assess if stromal fibroblasts express syndecan-1.
Design: Serial samples of normal lip (n=12) and lip SCC (n=11) biopsies were immunostained for syndecan-1 and prolyl-4-hydroxylase (fibroblast marker) detection. Samples were digitalized and analyzed at the reticular and papillar areas of normal lip, and at the intratumoral (IT) and peritumoral (PT) stroma of lip SCC to obtain syndecan-1 expression score and fibroblast density, and to assess co-localization of fibroblasts and syndecan-1.
Results: Both syndecan-1 expression and fibroblast density were increased in lip SCC as compared to normal lip (P<0.001, Wilcoxon). In addition, fibroblasts from normal lip samples showed no syndecan-1 expression (0/11), whereas, 8 out of 11 samples of lip SCC showed syndecan-1 expression by stromal fibroblasts (P<0.0001, Fisher test).
Conclusions: The results showed that fibroblast density was significantly increased in lip SCC as compared to normal lip. In addition, fibroblasts from normal lip did not express syndecan-1, whereas, stromal fibroblasts from lip SCC showed increased syndecan-1 expression . This suggests that stromal fibroblasts have and activated phenotype in malignant lip lesions that could contribute to lip cancer growth and invasion. Supported by CONICYT, grant FONDECYT 1090287.
Category: Head & Neck
Tuesday, March 23, 2010 1:00 PM
Poster Session IV # 150, Tuesday Afternoon