IMP2, an Adjunct Marker in the Diagnosis and Grading of Cervical Intraepithelial Neoplasia
L Zhang, Y Liu, D Lu. University of Massachusetts, Worcester, MA
Background: Accurate diagnosis and grading of cervical intraepithelial neoplasia (CIN) play a key role in the cervical cancer prevention. But it has met with difficulty and inconsistency in pathology practice. Biomarkers, such as p16 and ki-67, have shown to be very helpful. Here we present data of a new biomarker, IMP2 (insulin-like growth factor II mRNA-binding protein 2), and its immunohistochemical stain in CIN lesions. IMP2 is a member of family of three components, IMP1, 2, and 3. In this article, we demonstrate that IMP2 staining identifies CIN, and the staining pattern correlates with CIN's grade.
Design: The total 254 biopsies are selected from the specimen database at Department of Pathology, University of Massachusetts between 1997 and 1998, including benign (n=76), CIN-1 (n=25), CIN-2 (n=43), and CIN-3 (n=186). All cases had follow up LEEP or hysterectomy to confirm the diagnoses. The conventional H&E and IMP2 immunohistochemical stains are performed on consecutive tissue sections. The staining method is used routinely in our lab and published elsewhere. Positive is defined as dark brown cytoplasmic staining and the patterns are defined in concordance with CIN grading. Scant fine granular background staining, or no staining at all is considered negative.
Results: 1. IMP2 immunohistochemical stains of benign and CIN lesions:
Figure 1 demonstrate the different IMP2 staining patterns in benign, CIN-1, -2 and -3 lesions. 2. IMP2 staining pattern is adjunct tool in CIN grading:
Figure 2 illustrate the positivity of IMP2 staining in CIN lesions (in comparision to benign lesions); it also illustrate the positive correlation between the IMP2 staining and the patterns of dysplasia among different CIN lesions.
Conclusions: Our study indicates positive IMP2 stain identifies CIN. Its staining pattern correlates with CIN's grades. IMP2 can be a useful adjunct in diagnosis and grading of CIN lesions.
Category: Gynecologic & Obstetrics
Tuesday, March 23, 2010 9:30 AM
Poster Session III # 176, Tuesday Morning