E2F-1 Expression Correlates with p16 in Cervical Dysplasias
WU Todd, JF Silverman. Allegheny General Hospital, Pittsburgh, PA
Background: E2F-1 is a nuclear transcription factor that binds with pRB during the regulation of several genes needed for cell cycle entry and DNA synthesis. The expression of E2F-1 has not been reported in cervical dysplasia.
Design: 72 cases of normal cervix and cervical dysplasia were retrieved for immunohistochemical analysis. The cases encompassed 8 normal, 24 cervical intraepithelial neoplasia (CIN) I, 21 CIN II and 22 CIN III. Immunohistochemical analysis was performed using antibodies directed against p16 and E2F-1. Positive staining was quantitatively and qualitatively recorded. The fisher exact test was used to determine significance.
Results: Normal biopsies of cervical squamous epithelium predominantly showed a uniform staining pattern along the basal layers and more superficial layers of the epithelium. Higher grade dysplastic epithelium (CIN II and III) showed a loss of this uniform pattern with E2F-1 staining became weaker and more sporadic (P<0.01). The loss of the uniform pattern also correlated with p16 expression (P<0.001).
Conclusions: There are qualitative differences between higher grade cervical dysplasias (CIN II and CIN III) and normal/CIN I cervical biopsies. This change in staining pattern also correlated well with p16 expression in cervical biopsies. These findings may indicate a role for E2F-1 in the progression to higher grade cervical dysplasias and help in the differentiation of CIN I from higher grade dysplasias.
Category: Gynecologic & Obstetrics
Tuesday, March 23, 2010 9:30 AM
Poster Session III # 177, Tuesday Morning