Analysis of Human Papillomavirus 16E6 Oncogene Mutation in Cervical Cancer
F Sun, X Ha, C Xing, X Cao. The 2nd People's Hospital of Zhuhai, Zhuhai, Guangdong, China; General Hospital of PLA Lanzhou, Lanzhou, Gansu, China; General Hospital of PLA Lanshou, Lanzhou, Gansu, China
Background: Cervical cancer (CC) is the second most common type of cancer in women worldwide, after breast cancer. High-risk human papillomaviruses (HR-HPVs) are considered to be major causes of cervical cancer. HPV16 is the most common type of HR-HPVs and HPV16 E6 gene is one of major oncogenes. Specific mutations are considered as dangerous factors causing CC. In recent studies the mutations of HPV16 E6 gene and the protein coded by it are considered to have a direct relationship with the development of CC. The mutation of HPV16 E6 is different with respect to the different geographical regions over the globe. This study was designed to find mutations of HPV16 E6 and the relationship between the mutations and the happening of Chinese CC population.Current work aimed at finding this gene mutations and its relationship between mutations and happening of CC.
Design: The tissue DNA was extracted from 15 CC biopsies. Part of HPV16 E6 gene (nucleotide 201-523) was amplified by polymerase chain reaction (PCR) from the CC tissue DNA. The PCR fragments were sequenced and analyzed. Collect 15 CC specimens (age range, 24-65 years; mean age, 41 years) from a Maternal and Child Health Hospital from 2007 to 2008. All patients didn't receive radiotherapy or chemiotherapy. All cases were reviewed and diagnosed as CC by 3 associate chief physicians of pathology department. And the specimens were stored in -60° C freezer.
Results: The result of PCR showed that the positive rate of HPV16 E6 was 93.33% (14/15). After sequencing and analyzing the 13 of 14 PCR fragments we found that 4 of them maintained prototype (30.77%), 8 have a same 350G mutation (61.54%), and 1 has a 249G mutation (7.69%).
Conclusions: Our findings suggested that HPV16 E6 is the highly occurring gene mutation. The mutations of it may shed light on the development of cervical cancer. Further work is needed to disclose the new found 249G mutation.
Category: Gynecologic & Obstetrics
Tuesday, March 23, 2010 9:30 AM
Poster Session III # 190, Tuesday Morning