HPV Genotyping and HPV16 Variant Analysis in Glandular and Squamous Neoplastic Lesions of the Uterine Cervix
KD Quint, MNC de Koning, L-J van Doorn, WGV Quint, EC Pirog. DDL Diagnostic Laboratory, Voorburg, Netherlands; Weill Medical College of Cornell University, New York
Background: The goal of the study was to compare the distribution of HPV16 genotypic variants in glandular and squamous neoplasia of the uterine cervix. The incidence of cervical adenocarcinoma is increasing and since the reason for this phenomenon is not known, a detailed HPV variant analysis in cervical neoplasia is of interest.
Design: The study consisted of 278 cases including endocervical adenocarcinoma in-situ (AIS, n=33) invasive adenocarcinoma (ADCA, n=55), cervical intraepithelial neoplasia-3 (CIN3, n=130) and squamous cell carcinoma (SCC, n=60) collected at the New York - Presbyterian Hospital in New York. All cases were tested for HPV by SPF10PCR-LIPA25 (version1) genotyping assay and cases positive for HPV16 were further tested for intratypic variants using a multiplex PCR and reverse hybridization assay.
Results: HPV DNA was detected in all cases with the exception of one case of CIN3 and three cases of ADCA. There was a significant difference in the spectrum of HPV genotypes identified in the glandular and squamous neoplastic lesions: while as much as 13 different HPV genotypes were detected in CIN3 as single infections and 11 types were found in SCC, only 4 genotypes were detected in AIS and 3 in ADCA. The most common single HPV types in CIN3 were HPV16, 31, and 52 (56.9%, 10%, 8.4%, respectively). In SCC the most common were HPV16, 18 and 31 (70%, 6.5%, 4.9%). In AIS, HPV16, 18, 45 and 35 accounted for 69.7%, 27.2%, 3%, 3% of cases. The 3 single types in ADCA were HPV16 (43.6%), HPV18 (41.8%) and HPV45 (10.9%). There were significant differences in prevalence of HPV16 variants between the squamous and glandular neoplastic lesions. The European variants of HPV16 were the most common in CIN3 (83.8%), SCC (71.4%) and AIS (73.9%). In ADCA the Asian American (AA) variant of HPV16 was the most common (41.7%) followed by the European variants (33.3%). Overall, AA variant of HPV16 was detected in 2.3% of all CIN3, 1.6% of all SCC, 12.1% of all AIS and 18.2% of all ADCA.
Conclusions: Asian American variant of HPV16, HPV18 and HPV45 are preferentially associated with in-situ and invasive cervical adenocarcinoma.
Category: Gynecologic & Obstetrics
Tuesday, March 23, 2010 9:30 AM
Poster Session III # 181, Tuesday Morning